Toxic effects of resazurin on cell cultures
Toxic effects of resazurin on cell cultures
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DOI：
10.1007/s10616-013-9664-1
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发表时间：
2015-01-01
期刊：
影响因子：
2.2
通讯作者：
Burg, Karen Jane Leadbetter
中科院分区：
文献类型：
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作者：
Pace, Richard Thomas;Burg, Karen Jane Leadbetter
刃天青作为细胞活力指示剂以商品名alamarBlue（A（R））引入，当根据制造商建议的短期孵育时间规格使用时，通常认为其无毒。然而，当暴露时间延长到数天的长期培养时，就会出现问题。为了评估刃天青在较长孵育时间内的作用，在4天和8天期间用不同量的刃天青检测了MCF 7（HTB-22）、MCF 10A（CRL-10317）、3 T3-L1（CL-173）和D1（CRL-12424）培养物。用20%alamarBlue（A（R））培养8天的MCF 7、3 T3-L1和D1细胞具有显著更低的细胞存活率。具体而言，在含有20% alamarBlue（A（R））的培养基中培养8天的细胞系的代谢活性、脱氧核糖核酸（DNA）浓度和葡萄糖消耗水平显著低于（p < 0.05）在不含alamarBlue（A（R））的培养基中培养8天的MCF 7细胞的代谢活性、DNA浓度和葡萄糖消耗。MCF 7、3 T3-L1和D1细胞在低至5%的浓度下使用较少的葡萄糖。数据还表明，与非癌细胞相比，癌细胞系中的毒性作用更明显。
Resazurin, introduced as a cell viability indicator under the trade name alamarBlue(A (R)), is generally regarded as nontoxic when used according to manufacturer's suggested shorter-term incubation time specifications. However, problems arise when exposure times are extended to longer-term cultures on the order of days. To assess the effect of resazurin over longer incubation times, MCF7 (HTB-22), MCF10A (CRL-10317), 3T3-L1 (CL-173), and D1 (CRL-12424) cultures were tested with varying amounts of resazurin over 4- and 8-day periods. MCF7, 3T3-L1, and D1 cells cultured for 8 days with 20 % alamarBlue(A (R)) had significantly less cell survivability. Specifically, levels of metabolic activity, deoxyribonucleic acid (DNA) concentration, and glucose consumption of the cell lines cultured for 8 days in medium with 20 % alamarBlue(A (R)) were significantly lower (p < 0.05) than metabolic activity, DNA concentration, and glucose consumption of MCF7 cells cultured for 8 days in medium with no alamarBlue(A (R)). MCF7, 3T3-L1, and D1 cells used less glucose at concentrations as low as 5 %. Data also suggests the toxic effects are more pronounced in the cancerous cell line as compared to the noncancerous cells.