Genetic mapping of eight SH3 domain genes on seven mouse chromosomes.
Genetic mapping of eight SH3 domain genes on seven mouse chromosomes.
复制标题
七个小鼠染色体上八个 SH3 结构域基因的遗传图谱。
DOI：
10.1007/s003359901011
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Kozak,CA
中科院分区：
文献类型：
--
作者：
Lee,CG;Morse3rd,HC;Kay,BK;Kozak,CA
用噬菌体优化的肽配体筛选 cDNA 表达文库的过程，称为配体靶标克隆 (COLT)，最近用于分离一系列含有 SH3 结构域的蛋白质 (Sparks 等人，1996)。在鉴定出的 18 种含有 SH3 结构域的蛋白质中，有 9 种以前从未在小鼠中报告过。本研究中绘制基因图谱的七种蛋白质的结构如图 1 所示。许多实验室正在积极研究许多小鼠蛋白质的功能。其中三种蛋白质 SH3P4、SH3P8 和 SH3P14 在结构上高度相关，并且已被发现可结合突触蛋白和动力蛋白，并参与内吞作用（de Heuvel 等人，1997 年；Ringstad 等人，1997 年）。该蛋白质家族也在人类中被发现（Giachino 等人，1997 年），被称为内亲素 1、2 和 3。SH3P8 还被鉴定为与人类急性髓性白血病中的 MLL 融合的基因（So 等人，1997 年），以及在酵母双杂交筛选中与鼠白血病病毒的 Gag 蛋白结合的蛋白质（W. Kim， T. Torrey、H. Morse，未发表的观察结果）。 SH3P9 因其与两栖蛋白的高度相似性而被重新命名为两栖蛋白 II，已被证明是内吞机制的一个组成部分（Butler 等人，1997 年；Ramjaun 等人，1997 年）以及细胞骨架的一个组成部分，它可以在细胞骨架中调节 c-Abl 酪氨酸激酶（Kadlec 和 Pendergast，1997 年）。据观察，SH3P12 与细胞中的 c-Cbl 相互作用，它可能在信号转导途径和细胞骨架的调节中发挥作用（Ribon 等人，1998）。为了更多地了解七种新型小鼠蛋白质的基因，我们着手绘制它们的染色体位置。分析两组多位点遗传杂交编码SH3家族基因的小鼠基因的遗传：（NFS/N或C58/J×
The process of screening cDNA expression libraries with phageoptimized peptide ligands, termed cloning of ligand targets (COLT), was recently used to isolate a series of SH3 domaincontaining proteins (Sparks et al. 1996). Among the 18 SH3 domain-containing proteins identified, nine were previously unreported from mouse. The structures of seven of the proteins whose genes are mapped in this study are diagrammed in Fig. 1. The function of many of the mouse proteins is being actively pursued by many laboratories. Three of the proteins, SH3P4, SH3P8, and SH3P14, are highly related in structure and have been discovered to bind synaptojanin and dynamin, and to be involved in endocytosis (de Heuvel et al. 1997; Ringstad et al. 1997). This family of proteins, which has also been discovered in human (Giachino et al. 1997), has been termed endophilin 1, 2, and 3. SH3P8 was also identified as a gene fused to MLL in human acute myeloid leukemia (So et al. 1997) and as a protein that binds to the Gag protein of murine leukemia viruses in a yeast two-hybrid screen (W. Kim, T. Torrey, H. Morse, unpublished observations). SH3P9, renamed amphiphysin II because of its strong similarity to amphiphysin, has been shown to be a component of the endocytic machinery (Butler et al. 1997; Ramjaun et al. 1997) as well as the cytoskeleton, where it may regulate the c-Abl tyrosine kinase (Kadlec and Pendergast 1997). SH3P12 has been observed to interact with c-Cbl in cells where it may play a role in both signal transduction pathways and regulation of the cytoskeleton (Ribon et al. 1998). To learn more about the genes for the seven novel mouse proteins, we set out to map their chromosomal locations. Two sets of multilocus genetic crosses were analyzed for inheritance of the mouse genes encoding the SH3 family genes:(NFS/N or C58/J×