Application of two-dimensional electrophoresis without denaturing agents to comparative study of vertebrate plasma proteins
Application of two-dimensional electrophoresis without denaturing agents to comparative study of vertebrate plasma proteins
复制标题
无变性剂二维电泳在脊椎动物血浆蛋白比较研究中的应用
DOI：
10.2198/sbk.42.13
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
T. Manabe
中科院分区：
文献类型：
--
作者：
Y. Shimazaki;T. Manabe
为了了解不同脊椎动物血浆蛋白组成的差异，采用无变性双向电泳（2-D PAGE）技术对脊椎动物血浆蛋白进行了比较研究。对来自浣熊、鸡、龟、蝾螈、金鱼和人的血浆进行分析。2-D PAGE图谱提供了脊椎动物血浆蛋白在生理条件下的信息：它们的等电点（pI）、近似分子量（M。W.）和数量。每种脊椎动物血浆蛋白都表现出特征性的蛋白质分布，反映了DNA序列的进化变化和血浆蛋白的表达。另一方面，对于某些血浆蛋白，免疫化学上表明了人与其他脊椎动物之间的结构关系。在pI 6.2-8.3检测到一系列斑点，M. W.用抗人免疫球蛋白M抗体对浣熊、鸡和鳖血浆的2-D PAGE图谱进行免疫化学染色。在pI4.8 -5.5范围内检测到一系列斑点，M. W. 500-用抗人α2-巨球蛋白抗体对浣熊、鸡和蝾螈血浆中的kDa进行免疫组化染色。这些结果表明，用无变性剂的2-D聚丙烯酰胺凝胶电泳可以获得不同脊椎动物血浆蛋白组成的变化。
A comparative study of vertebrate plasma proteins was performed by two-dimensional electrophoresis (2-D PAGE) without denaturing agents in order to obtain a perspective of the variation of plasma protein composition in different vertebrates. Plasma from raccoon, chicken, turtle, newt, goldfish and human was subjected to the analysis. The 2-D PAGE patterns offered information on vertebrate plasma proteins in their physiological conditions: their isoelectric point (pI), approximate molecular weight (M. W.) and quantity. Each vertebrate plasma protein showed a characteristic protein distribution, reflecting the evolutionary changes in DNA sequence and the expression of plasma protein. On the other hand, for some plasma proteins, the structural relationships between human and other vertebrates were suggested immunochemically. A series of spots detected at pI 6.2-8.3, M. W. 850-kDa, observed in the 2-D PAGE patterns of raccoon, chicken and turtle plasma was immunochemically stained by antihuman immunoglobulin M antibody. And, a series of spots detected at pI 4.8-5.5, M. W. 500-kDa in the patterns of raccoon, chicken and newt plasma was immunochemically stained by anti-human α2-macroglobulin antibody. These results indicate that a perspective of the variation of plasma protein composition in different vertebrates may be obtained by 2-D PAGE without denaturing agents.