Effects of lactic acid bacteria inoculation in pre-harvesting period on fermentation and feed quality properties of alfalfa silage
Effects of lactic acid bacteria inoculation in pre-harvesting period on fermentation and feed quality properties of alfalfa silage
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DOI：
10.5713/ajas.18.0801
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发表时间：
2020-02-01
期刊：
影响因子：
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通讯作者：
Kizilsimsek, Mustafa
中科院分区：
文献类型：
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作者：
Ertekin, Ibrahim;Kizilsimsek, Mustafa
【摘要】：目的:为研究苜蓿(Medicago sativa Lam.)青贮饲料的发酵品质和化学成分,在收获前24小时在田间接种不同菌株的乳酸菌(LAB)。方法:处理组为:以不添加添加剂的青贮饲料作为对照,接种短乳杆菌(LS-55-2-2)、柠檬明串珠菌(L. citerum; L-70-6-1)、双发酵乳杆菌(L. bifermentans；LS-65-2-1)、植物乳杆菌(L. plantarum；LS-3-3)和植物乳杆菌(LS-72-2)。所有青贮饲料均在25℃下储存，测定pH、微生物和挥发性脂肪酸含量、粗蛋白、中性洗涤纤维、酸性洗涤纤维、净气、代谢能、有机物消化率、干物质采食量和相对饲料值等参数，测定苜蓿青贮饲料的发酵品质、化学成分和相对饲料价值。结果：对照组和处理组在各开放时间的pH和微生物含量以及粗蛋白、净气、代谢能方面均存在显着差异。和最终青贮饲料的有机物消化率。所有处理的pH值范围为4.70至5.52，对照青贮饲料在T(75)d青贮饲料时具有总体处理的最高值。青贮饲料的挥发性脂肪酸不受接种的显着影响。然而，双发酵乳杆菌（LS-65-2-1）的乳酸含量高于其他处理。柠檬酸乳杆菌 (L-70-6-1) 接种记录了最高的代谢能和有机物消化率。此外，在中性洗涤纤维、酸性洗涤纤维、干物质摄入量和相对饲料价值方面，处理之间没有发现显着差异。结论：在处理的LAB分离物中，双发酵乳杆菌尤其在有机酸组成和青贮饲料的品质特性方面表现突出。
Objective: To develop the fermentation quality and chemical composition of alfalfa (Medicago sativa Lam.) silage, plants were inoculated with different lactic acid bacteria (LAB) strains at field 24 hours before harvest.Methods: The treatment groups were as follow: silage without additive as a control and inoculated with each strains of Lactobacillus brevis (LS-55-2-2), Leuconostoc citerum (L. citerum; L-70-6-1), Lactobacillus bifermentans (L. bifermentans; LS-65-2-1), Lactobacillus plantarum (L. plantarum; LS-3-3) and L. plantarum (LS-72-2). All the silages were stored at 25 degrees C. Parameters such as pH, microorganism and volatile fatty acid contents, crude protein, neutral detergent fiber, acid detergent fiber, net gas, metabolizable energy, organic matter digestibility, dry matter intake and relative feed value were measured to determine fermentation quality, chemical compositions and relative feed value of alfalfa silages.Results: Significant differences were found among the control and treated groups in terms of pH and microorganism contents at all opening times and crude protein, net gas, metabolizable energy and organic matter digestibility of final silage. The pH values ranged from 4.70 to 5.52 for all treatments and control silage had the highest value of overall treatments at T(75)d silages. Volatile fatty acid of silages was not influenced significantly by inoculations. However, lactic acid content of L. bifermentans (LS-65-2-1) was higher than the other treatments. The highest metabolizable energy and organic matter digestibility were recorded from L. citerum (L-70-6-1) inoculation. In addition, no significant differences were found among treatments in terms of neutral detergent fiber, acid detergent fiber, dry matter intake and relative feed value.Conclusion: Among the treated LAB isolates, L. bifermentans came into prominence especially in terms of organic acid composition and quality characters of silages.