trans-dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes.
trans-dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes.
复制标题
对人类免疫缺陷病毒 1 型 Rev 的反式显性抑制是通过形成无活性的蛋白质复合物而发生的。
DOI：
10.1128/jvi.66.4.1849-1855.1992
复制
发表时间：
1992
影响因子：
5.4
通讯作者：
Parslow,TG
中科院分区：
文献类型：
--
作者：
Hope,TJ;Klein,NP;Elder,ME;Parslow,TG
The human immunodeficiency virus type 1 Rev protein controls expression of certain viral RNAs by binding to these RNAs in the nucleus. To investigate how dominant negative Rev mutants inhibit Rev function, we fused such mutants to hormone-dependent localization signals from the glucocorticoid receptor. Each was found to have fully potent inhibitory activity whether expressed in the nucleus or in the cytoplasm. Wild-type Rev colocalized with an inhibitory fusion protein, implying that the two proteins interact. The resulting complexes accumulated within nuclei in response to steroids but had no effect on expression of Rev-responsive mRNAs. A mutation known to block in vitro oligomerization of Rev abolished both complex formation and inhibitory activity of the mutant fusion proteins. Thus, trans-dominant inhibition of Rev does not require competition for nuclear substrates but may instead reflect the ability of a mutant to form nonfunctional complexes with the wild-type protein in vivo.
