Aqueous Humor Dynamics of the Brown-Norway Rat
Aqueous Humor Dynamics of the Brown-Norway Rat
复制标题
褐-诺威大鼠的水液动力学
DOI：
10.1167/iovs.17-22915
复制
发表时间：
2018-05-01
影响因子：
4.4
通讯作者：
Passaglia, Christopher L.
中科院分区：
文献类型：
--
作者：
Ficarrotta, Kayla R.;Bello, Simon A.;Passaglia, Christopher L.
目的。本研究旨在提供健康大鼠眼房水动力学的定量描述。方法：将26只成年Brown-挪威大鼠麻醉后的一只眼用针连接到灌注泵和压力传感器上。通过改变泵速(恒流法)或调节泵占空比使眼压(IOP)保持在设定水平(改良恒压法)，测量活眼和死眼的压力-流量数据。用Goldmann方程对数据进行拟合，估算出常规流出量(C)和非常规流出量(FUN)。分别通过向眼内插入类似电导的分流管和采用不同的眼部水化方法来检查参数估计。结果：大鼠静息状态下的平均眼压为14.6+/-1.9 mm Hg。两种灌流技术的压力-流量数据具有可重复性和不可区分性，分别为C=0.023+/-0.002亩L/分钟/毫米汞和FUN=0.096+/-0.024 LL/分钟。C对于活眼和死眼是相似的，并且在分流插入时增加了等于分流电导的量，验证了测量的准确性。在100%湿度下，FUN降至0.003+/-0.030 LL/分钟。没有观察到生理冲洗(-0.35+/-0.65%/h)，小梁解剖结构看起来正常。结论：与小鼠和人类相比，大鼠房水动力学程度中等，与眼睛大小的物种差异一致。C不会随着时间或死亡而改变。即使在眼睛没有被摘除的情况下，蒸发也会使趣味的测量变得复杂。到目前为止，只有老鼠和人的眼睛才能看到一个值得注意的发现，即没有洗涤。
PURPOSE. The study aimed to provide a quantitative description of aqueous humor dynamics in healthy rat eyes.METHODS. One eye of 26 anesthetized adult Brown-Norway rats was cannulated with a needle connected to a perfusion pump and pressure transducer. Pressure-flow data were measured in live and dead eyes by varying pump rate (constant-flow technique) or by modulating pump duty cycle to hold intraocular pressure (IOP) at set levels (modified constant-pressure technique). Data were fit by the Goldmann equation to estimate conventional outflow facility (C) and unconventional outflow rate (Fun). Parameter estimates were respectively checked by inserting a shunt of similar conductance into the eye and by varying eye hydration methodology.RESULTS. Rat IOP averaged 14.6 +/- 1.9 mm Hg at rest. Pressure-flow data were repeatable and indistinguishable for the two perfusion techniques, yielding C =0.023 +/- 0.002 mu L/min/mm Hg and Fun = 0.096 +/- 0.024 lL/min. C was similar for live and dead eyes and increased upon shunt insertion by an amount equal to shunt conductance, validating measurement accuracy. At 100% humidity Fun dropped to 0.003 +/- 0.030 lL/min. Physiological washout was not observed (-0.35 +/- 0.65%/h), and trabecular anatomy looked normal.CONCLUSIONS. Rat aqueous humor dynamics are intermediate in magnitude compared to those in mice and humans, consistent with species differences in eye size. C does not change with time or death. Evaporation complicates measurement of Fun even when eyes are not enucleated. Absence of washout is a notable finding seen only in mouse and human eyes to date.