Cloning of a fragment of the osteonectin gene from goldfish, Carassius auratus: its expression and potential regulation by estrogen.
Cloning of a fragment of the osteonectin gene from goldfish, Carassius auratus: its expression and potential regulation by estrogen.
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金鱼骨连接蛋白基因片段的克隆，鲫鱼：其表达和雌激素的潜在调节。
DOI：
10.1006/gcen.1998.7237
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发表时间：
1999
影响因子：
2.7
通讯作者：
I. Henderson
中科院分区：
文献类型：
--
作者：
D. Lehane;N. Mckie;R. Russell;I. Henderson
在繁殖过程中，雌性硬骨鱼显示增加血浆雌激素和大大增加总血浆钙浓度，这种钙的主要来源似乎是规模。骨连接素是一种胶原结合糖蛋白，是哺乳动物骨的主要非胶原成分，主要是成骨细胞的产物。应用RT-PCT技术对金鱼骨粘连蛋白基因进行了克隆和部分序列测定。使用金鱼鳞片细胞系（GFS）和金鱼骨粘连蛋白mRNA的特异性探针，可以研究雌激素和其他促钙激素对来自鳞片的细胞的潜在影响。通过北方印迹分析，在硬骨鱼骨、鳞和GFS细胞中检测到骨连接素mRNA，与约1.6 kb的转录物杂交。17 β-雌二醇（10（-8）至10（-11）M）以剂量依赖性方式显著下调骨连接蛋白mRNA的表达，但不受骨化三醇、TGF β、IL-1 β、降钙素和PTHrP的影响。雌激素下调骨连接蛋白进一步证明雌激素参与卵黄发生过程中的钙稳态，直接作用于鳞片中负责基质和矿物质通量的细胞。
During reproduction, female teleost fish display increased plasma estrogen and greatly increased total plasma calcium concentrations; the main source of this calcium seems to be the scale. Osteonectin, a collagen-binding glycoprotein, is a major noncollagenous constituent of mammalian bone and is a product mainly of the osteoblasts. RT-PCT has been applied to clone and sequence part of the osteonectin gene from the goldfish, Carassius auratus. The use of a goldfish scale cell line (GFS) and a specific probe to goldfish osteonectin mRNA has allowed the study of the potential effects of estrogen and other calcitropic hormones on the cells derived from the scales. Osteonectin mRNA was detected in teleost bone, scale, and GFS cells by Northern blot analysis, hybridising to a transcript of approximately 1.6 kb. Expression of osteonectin mRNA was markedly down-regulated by 17beta-estradiol (10(-8) to 10(-11) M) in a dose-dependent fashion but was unaffected by calcitriol, TGFbeta, IL-1beta, calcitonin, and PTHrP. Down-regulation of osteonectin by estrogen is further evidence that estrogen participates in calcium homeostasis during vitellogenesis, acting directly on the cells responsible for matrix and mineral fluxes in scales.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
在矿化鳞片中发现骨γ-羧基谷氨酸蛋白。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nishimoto,SK;Araki,N;Robinson,FD;Waite,JH
通讯作者：
Waite,JH
鸡胚 SPARC（骨连接素）的分子分析。
DOI：
10.1111/j.1432-1033.1993.tb18358.x
发表时间：
1993
期刊：
European journal of biochemistry
影响因子：
--
作者：
Bassuk,JA;Iruela-Arispe,ML;Lane,TF;Benson,JM;Berg,RA;Sage,EH
通讯作者：
Sage,EH