The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices.
The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices.
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DOI：
10.1016/j.biomaterials.2012.06.057
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发表时间：
2012-10
期刊：
影响因子：
14
通讯作者：
Engler, Adam J.
中科院分区：
文献类型：
--
作者：
Choi, Yu Suk;Vincent, Ludovic G.;Lee, Andrew R.;Kretchmer, Kyle C.;Chirasatitsin, Somyot;Dobke, Marek K.;Engler, Adam J.
细胞图案化通常是通过选择性地将用于细胞粘附的蛋白质沉积在图案化区域来完成的；然而，在组织中，细胞也受到机械刺激的影响，这也可以导致细胞的图案排列。我们开发了一种机械图案水凝胶来观察并将其与细胞外基质（ECM）配体图案进行比较，以确定如何最好地调节和改善细胞类型特异性行为。在长时间的培养过程中，水凝胶上基于配体的模式并不稳固，但机械模式水凝胶上的细胞根据刚度偏好进行了不同的分类：肌细胞和脂肪来源的干细胞（ASCs）经历了刚度介导的迁移，即刚性迁移，并停留在肌源性水凝胶区域。肌细胞发育成排列的条纹，并在机械图案水凝胶的肌原条纹上融合。ASCs排列整齐并发生肌发生，但它们的融合率增加了，由于它们排列整齐，融合成肌管的细胞数量也增加了。相反，神经细胞不表现出硬性，并且可以在水凝胶的软区看到长时间的培养。这些结果表明，机械图案水凝胶可以提供一个平台，以创建组织工程，神经和肌肉表型相邻并列的受神经支配的微肌肉，以便更好地重建肌肉生态位。
Cell patterning is typically accomplished by selectively depositing proteins for cell adhesion only on patterned regions; however in tissues, cells are also influenced by mechanical stimuli, which can also result in patterned arrangements of cells. We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand patterns to determine how to best regulate and improve cell type-specific behaviors. Ligand-based patterning on hydrogels was not robust over prolonged culture, but cells on mechanically-patterned hydrogels differentially sorted based on stiffness preference: myocytes and adipose-derived stem cells (ASCs) underwent stiffness-mediated migration, i.e. durotaxis, and remained on myogenic hydrogel regions. Myocytes developed aligned striations and fused on myogenic stripes of the mechanically-patterned hydrogel. ASCs aligned and underwent myogenesis, but their fusion rate increased, as did the number of cells fusing into a myotube as a result of their alignment. Conversely, neuronal cells did not exhibit durotaxis and could be seen on soft regions of the hydrogel for prolonged culture time. These results suggest that mechanically-patterned hydrogels could provide a platform to create tissue engineered, innervated micro-muscles of neural and muscle phenotypes juxtaposed next to each other in order better recreate a muscle niche.
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影响因子：
14
作者：
Choi, Yu Suk;Matsuda, Ken;Dilley, Rodney J.
通讯作者：
Dilley, Rodney J.
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE
DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Discher DE;Mooney DJ;Zandstra PW
通讯作者：
Zandstra PW