Transcriptomic responses to aluminum stress in soybean roots.
Transcriptomic responses to aluminum stress in soybean roots.
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DOI：
10.1139/g11-060
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发表时间：
2011-10
期刊：
影响因子：
3.1
通讯作者：
J. You;Hongmei Zhang;Ning Liu;Lingling Gao;L. Kong;Zhen-ming Yang
中科院分区：
文献类型：
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作者：
J. You;Hongmei Zhang;Ning Liu;Lingling Gao;L. Kong;Zhen-ming Yang
铝毒害是酸性土壤上作物生产和植物生长的主要限制因素。大豆具有多种抗铝机制，包括铝诱导的柠檬酸分泌对根尖中铝的络合和排斥作用。基因芯片分析可用于大豆基因的鉴定。本研究利用Affymetrix大豆基因组芯片，筛选出抗铝品种冀育70中铝诱导差异表达的基因。在非铝处理和铝处理根尖之间的截止值为>；2.0倍(p<；0.05)的情况下，30μ/AlCl3处理4h后，有561个基因上调，78个基因下调。分析表明，近一半的铝反应基因具有未知的生物学功能。在铝诱导的上调基因和下调表达的基因中，转录调控相关基因和细胞壁过程相关基因的比例分别较高。在我们的分析中发现了一些与柠檬酸转运蛋白Mate家族基因或C(2)H(2)家族转录因子基因STOP1同源的基因。一些与木质素沉积相关的基因表达上调，可能与铝诱导的根伸长抑制有关。
Aluminum (Al) toxicity is the primary limitation to crop production and plant growth in acid soils. Soybean has multiple mechanisms of Al resistance including the complexing and exclusion of Al in root apices by Al-induced citrate secretion. Microarray analysis is available for the identification of genes in soybean. In the present study, Affymetrix soybean genome array was used to identify the Al-induced differentially expressed genes in Al-resistant genotype Jiyu 70. With a cutoff of > 2.0-fold (p < 0.05) between non Al-treated and Al-treated root apices, 561 genes were upregulated and 78 genes were downregulated when roots were exposed to 30 μmol/L AlCl(3) for 4 h. Quantitative real-time PCR was used to test the microarray data. The analysis showed that nearly half of the Al-responsive genes were of unknown biological function. A higher proportion of genes related to transcription regulation and cell wall processes were observed in Al-induced up- and downregulated genes, respectively. Some genes homologous to the citrate transporter MATE family gene or C(2)H(2) family transcription factor gene, STOP1, were detected in our analysis. Some genes related to lignin deposition were upregulated, which might be related to Al-induced root elongation inhibition.