InterB multigenic family, a gene repertoire associated with subterminal chromosome regions of Encephalitozoon cuniculi and conserved in several human-infecting microsporidian species
InterB multigenic family, a gene repertoire associated with subterminal chromosome regions of Encephalitozoon cuniculi and conserved in several human-infecting microsporidian species
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DOI：
10.1007/s00294-006-0114-x
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发表时间：
2007-03-01
期刊：
影响因子：
2.5
通讯作者：
Cornillot, Emmanuel
中科院分区：
文献类型：
--
作者：
Dia, Ndongo;Lavie, Laurence;Cornillot, Emmanuel
微孢子虫是与真菌相关的专性细胞内寄生虫，可感染包括人类在内的许多动物。兔脑炎虫拥有非常小的基因组 (2.9 Mbp)，包含约 2,000 个编码序列 (CDS)。大多数重复的 CDS 功能未知，分布在标记亚端粒 rDNA 单位和染色体核心之间过渡的亚末端区域。通过 PCR 和 RT-PCR 研究了编码大小范围为 579-641 aa 的蛋白质的潜在多基因家族 (interB)。最终，30 名成员被分配到 E. cuniculi interB 家族，并且发现主要的 interB 转录物源自第三号染色体上新鉴定的基因。对感染昆虫、鱼类或哺乳动物的八个不同属的微孢子虫物种进行了测试，以了解 interB 基因可能的门内保守性。只有脑炎虫属、玻璃虫属和臂尾虫属的代表呈阳性，这些属的宿主范围不同，但都能够侵入人类。 Brachiola algerae 的分子核型分析显示了一组复杂的染色体带，提供了 15-20 Mbp 的单倍体基因组大小估计。尽管基因组复杂性存在巨大差异，但 B. algerae 和 E. cuniculi 共享一些相似的 interB 基因拷贝以及 interB 基因在近 rDNA 亚末端区域的共同位置。
Microsporidia are fungi-related obligate intracellular parasites that infect numerous animals, including man. Encephalitozoon cuniculi harbours a very small genome (2.9 Mbp) with about 2,000 coding sequences (CDSs). Most repeated CDSs are of unknown function and are distributed in subterminal regions that mark the transitions between subtelomeric rDNA units and chromosome cores. A potential multigenic family (interB) encoding proteins within a size range of 579-641 aa was investigated by PCR and RT-PCR. Thirty members were finally assigned to the E. cuniculi interB family and a predominant interB transcript was found to originate from a newly identified gene on chromosome III. Microsporidian species from eight different genera infecting insects, fishes or mammals, were tested for a possible intra-phylum conservation of interB genes. Only representatives of the Encephalitozoon, Vittaforma and Brachiola genera, differing in host range but all able to invade humans, were positive. Molecular karyotyping of Brachiola algerae showed a complex set of chromosome bands, providing a haploid genome size estimate of 15-20 Mbp. In spite of this large difference in genome complexity, B. algerae and E. cuniculi shared some similar interB gene copies and a common location of interB genes in near-rDNA subterminal regions.