Molecularly imprinted polymer coated solid-phase microextraction fibers for determination of Sudan I-IV dyes in hot chili powder and poultry feed samples.
Molecularly imprinted polymer coated solid-phase microextraction fibers for determination of Sudan I-IV dyes in hot chili powder and poultry feed samples.
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DOI：
10.1016/j.chroma.2011.10.089
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发表时间：
2012-01
期刊：
影响因子：
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通讯作者：
Xiaogang Hu;Quanling Cai;Yanan Fan;Tingting Ye;Yujuan Cao;Chang-juan Guo
中科院分区：
文献类型：
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作者：
Xiaogang Hu;Quanling Cai;Yanan Fan;Tingting Ye;Yujuan Cao;Chang-juan Guo
本研究开发了一种以苏丹I为模板、不锈钢纤维为基材，大规模制备分子印迹聚合物(MIP)包覆固相微萃取纤维的新方法。在一根玻璃管中可以获得20根以上的光纤，与我们以前工作中只获得一根光纤的产率相比，效率和镀膜重复性都有了显著的提高。得到的MIP涂层不锈钢纤维具有涂层均匀、高度交联性、良好的化学稳定性和热稳定性、高的萃取量以及对苏丹I-IV染料的特殊选择性。在对萃取条件进行系统优化的基础上，建立了一种基于分子印迹聚合物包被的固相微萃取与高效液相色谱联用的简便、经济的方法，用于辣椒粉和家禽饲料样品中痕量苏丹I-IV染料的快速、选择性测定。苏丹I-IV染料的检出限为2.5~4.6 ng−1，加标回收率为86.3%~96.3%，家禽饲料样品的加标回收率为84.6%~97.4%。
In this research, a novel strategy was developed to prepare molecularly imprinted polymer (MIP) coated solid-phase microextraction fibers on a large scale with Sudan I as template and stainless steel fibers as substrate. More than 20 fibers could be obtained in one glass tube, and the efficiency and coating repeatability were enhanced remarkably in contrast with the yield of only one fiber in our previous works. The obtained MIP-coated stainless steel fibers were characterized by homogeneous and highly cross-linked coating, good chemical and thermal stabilities, high extraction capacities, and specific selectivities to Sudan I–IV dyes. Based on the systemic optimization of extraction conditions, a simple and cost-effective method based on the coupling of MIP-coated SPME with high-performance liquid chromatography was developed for the fast and selective determination of trace Sudan I–IV dyes in hot chili powder and poultry feed samples. The limits of detection of Sudan I–IV dyes were within 2.5–4.6ngg−1, and the spiked recoveries were in the range of 86.3–96.3% for hot chili powder sample and 84.6–97.4% for poultry feed sample.