Prognostic significance of serum microRNA-221 expression in human epithelial ovarian cancer
Prognostic significance of serum microRNA-221 expression in human epithelial ovarian cancer
复制标题
DOI：
10.1177/0300060513475759
复制
发表时间：
2013-02-01
影响因子：
1.6
通讯作者：
Zhu, Lin
中科院分区：
文献类型：
--
作者：
Hong, Fanzhen;Li, Yuyang;Zhu, Lin
目的：探讨血清微核糖核酸(MiR)-221作为卵巢上皮性癌(EOC)无创诊断和预后标志物的可行性。方法：采用实时定量逆转录-聚合酶链式反应(RT-PCR)检测卵巢上皮性癌(EOC)患者和健康对照组血清中miR-221的表达。结果：96例卵巢癌患者血清miR-221水平明显高于正常对照组(n=35)。血清miR-221表达水平与国际妇产科联合会分期、肿瘤分级显著相关。多因素生存分析显示，血清miR-221的高表达是卵巢癌一个独立的预后不良因素。结论：血清miR-221可能作为一种新的诊断和预后指标，有可能成为卵巢癌的治疗靶点。
Objective: To evaluate the feasibility of using serum microRNA (miR)-221 as a noninvasive diagnostic and prognostic biomarker in epithelial ovarian cancer (EOC).Methods: In this retrospective study, real-time reverse transcription-polymerase chain reaction was used to measure miR-221 expression in serum samples from patients with EOC and healthy age-matched controls. Correlations between serum miR-221 levels and clinicopathological factors and prognosis were explored.Results: Serum miR-221 was upregulated in patients with EOC (n = 96) compared with healthy controls (n = 35). The level of serum miR-221 expression was significantly associated with International Federation of Gynecology and Obstetrics stage, and tumour grade. Furthermore, multivariate analysis of overall survival showed that high serum miR-221 expression was an independent unfavourable prognostic factor in EOC.Conclusions: These findings indicate that serum miR-221 may have a role as a novel diagnostic and prognostic marker, and may have potential as a therapeutic target in EOC.