The zinc finger transcription factor Jing is required for dendrite/axonal targeting in Drosophila antennal lobe development.
The zinc finger transcription factor Jing is required for dendrite/axonal targeting in Drosophila antennal lobe development.
复制标题
锌指转录因子 Jing 是果蝇触角发育中树突/轴突靶向所必需的。
DOI：
10.1016/j.ydbio.2013.06.023
复制
发表时间：
2013
影响因子：
2.7
通讯作者：
K. VijayRaghavan
中科院分区：
文献类型：
--
作者：
I. Nair;Veronica Rodrigues;Veronica Rodrigues;H. Reichert;K. VijayRaghavan
嗅觉系统发育中的重要作用是由转录因子发挥的，这些转录因子在感觉神经元或其中间神经元靶中起作用，作为下游效应物的细胞自主调节剂，例如控制神经元身份和过程指导的细胞表面分子和信号系统。其中一些转录调节因子在果蝇嗅觉系统触角叶神经元的发育过程中得到了详细的研究。在这里，我们确定锌指转录因子Jing是一种自主作用的转录调节因子，它是果蝇嗅觉系统发育中投射神经元和局部中间神经元的树突靶向以及嗅觉感觉神经元的轴突靶向所需的。免疫细胞化学分析表明，Jing在胚后发育的神经细胞中广泛表达。基于MARCM的投射神经元和局部中间神经元谱系的克隆分析揭示了Jing在树突靶向中的需求; Jing功能丧失导致特定肾小球的神经支配丧失，不适当肾小球的异位神经支配，整个触角叶异常丰富的树突树枝状，以及对中枢神经系统其他部位的错误定位。ey-FLP-基于MARCM的嗅觉神经元分析揭示了Jing在轴突靶向中的额外需求; Jing的突变失活导致某些嗅觉感觉神经元轴突对DA 1肾小球的特异性错误定位，对其他肾小球的靶向减少，以及触角叶中轴突的异常停滞。综上所述，这些发现表明，Jing在果蝇发育中的嗅觉感觉系统中作为一个关键的转录控制元件。
An important role in olfactory system development is played by transcription factors which act in sensory neurons or in their interneuron targets as cell autonomous regulators of downstream effectors such as cell surface molecules and signalling systems that control neuronal identity and process guidance. Some of these transcriptional regulators have been characterized in detail in the development of the neural elements that innervate the antennal lobe in the olfactory system ofDrosophila. Here we identify the zinc finger transcription factor Jing as a cell autonomously acting transcriptional regulator that is required both for dendrite targeting of projection neurons and local interneurons as well as for axonal targeting of olfactory sensory neurons inDrosophilaolfactory system development. Immunocytochemical analysis shows that Jing is widely expressed in the neural cells during postembryonic development. MARCM-based clonal analysis of projection neuron and local interneuron lineages reveals a requirement for Jing in dendrite targeting; Jing loss-of-function results in loss of innervation in specific glomeruli, ectopic innervation of inappropriate glomeruli, aberrant profuse dendrite arborisation throughout the antennal lobe, as well as mistargeting to other parts of the CNS.ey-FLP-based MARCM analysis of olfactory sensory neurons reveals an additional requirement for Jing in axonal targeting; mutational inactivation of Jing causes specific mistargeting of some olfactory sensory neuron axons to the DA1 glomerulus, reduction of targeting to other glomeruli, as well as aberrant stalling of axons in the antennal lobe. Taken together, these findings indicate that Jing acts as a key transcriptional control element in wiring of the circuitry in the developing olfactory sensory system inDrosophila.
影响因子：
16
作者：
Cao, R;Zhang, Y
通讯作者：
Zhang, Y
Jing：slbo 的下游靶点，是边界细胞迁移发育控制所需的。
DOI：
10.1242/dev.128.3.321
发表时间：
2001
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Liu,Y;Montell,DJ
通讯作者：
Montell,DJ