Raman analysis of proteoglycans simultaneously in bone and cartilage
Raman analysis of proteoglycans simultaneously in bone and cartilage
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DOI：
10.1002/jrs.4552
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发表时间：
2014-09-01
影响因子：
2.5
通讯作者：
Paschalis, Eleftherios P.
中科院分区：
文献类型：
--
作者：
Gamsjaeger, Sonja;Klaushofer, Klaus;Paschalis, Eleftherios P.
骨和软骨是结缔组织，具有不同的有机基质（胶原和非胶原蛋白）组成，促进其生物学功能。蛋白聚糖（PG）是非胶原蛋白的一员，其功能由其核心蛋白和糖胺聚糖链决定。本研究的目的是确定代表PG浓度的拉曼谱带，并可用于骨和软骨组织。为了实现这一目标，我们分析了一系列参考PG和胶原蛋白，以及火鸡腿肌腱，以验证所识别的波段的激光偏振无关性。此外，通过拉曼成像和分层聚类分析在健康股骨头中测试了这些条带同时在软骨和骨组织中的适用性，以描述从关节软骨到软骨下骨的微米级PG分布。研究结果表明，与1375 cm（-1）相近的拉曼谱带可作为软骨和骨中PGs的标记谱带。此外，关节软骨具有较低的有机基质（主要是II型胶原）含量，而中间和深移行区具有较高浓度的PG。钙化软骨的特征是PG和总有机基质含量较低（根据酰胺III带的积分面积估计）。版权所有（c）2014约翰威利父子有限公司
Bone and cartilage are connective tissues with distinct organic matrix (collagen and non-collagenous proteins) composition facilitating their biological function. Proteoglycans (PGs), a member of the non-collagenous proteins fulfill functions that are determined by both their core protein and their glycosaminoglycan chains. The purpose of the present study was to identify Raman bands that are representative of PG concentration and may be used in both bone and cartilage tissues. To achieve this goal, we analyzed a series of reference PGs and collagens, as well as turkey leg tendon to verify the laser polarization independency of the identified bands. Additionally, the applicability of these bands in both cartilage and bone tissue simultaneously was tested in a healthy femoral head by Raman imaging and hierarchical cluster analysis to describe the distribution of PGs at the micron level from articular cartilage to subchondral bone. The results of the study show that the Raman band similar to 1375cm(-1) can be used as a PGs marker band in both cartilage and bone. Moreover, articular cartilage has a lower content of organic matrix (mostly type II collagen), while the middle and deep transitional zone haves a higher concentration of PGs. The calcified cartilage is characterized by a lower content of PGs and total organic matrix (estimated from the integrated area of the amide III band). Copyright (c) 2014 John Wiley & Sons, Ltd.