NOVEL MULTILAYERED LIPID VESICLES - COMPARISON OF PHYSICAL CHARACTERISTICS OF MULTILAMELLAR LIPOSOMES AND STABLE PLURILAMELLAR VESICLES
NOVEL MULTILAYERED LIPID VESICLES - COMPARISON OF PHYSICAL CHARACTERISTICS OF MULTILAMELLAR LIPOSOMES AND STABLE PLURILAMELLAR VESICLES
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DOI：
10.1021/bi00333a004
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
OSTRO, MJ
中科院分区：
文献类型：
--
作者：
GRUNER, SM;LENK, RP;OSTRO, MJ
本文报道了一种新型多层脂质体--稳定多层囊泡（SPLV）的制备。虽然SPLV和经典的多层囊泡（MLV）是由相同的材料制成，并出现明显相似的EM，2种类型的囊泡不同，确定的稳定性，包封率，ESR，NMR，X-射线衍射和生物效应。它表明，与已经假设的，经典MLV排除溶质在其形成过程中，因此，是在渗透压缩的状态下。相比之下，SPLV工艺产生的脂质体不被压缩。渗透压缩的影响进行了讨论。有人建议，渗透应力的状态是区分各种类型的脂质体，并具有显着的物理和生物学后果的一个重要变量。
The preparation of a new kind of multilayered liposome, called a stable plurilamellar vesicle (SPLV), is described. Although SPLV and classical multilamellar vesicles (MLV) are made of the same materials and appear overtly similar in the EM, the 2 types of vesicles differ as determined by stability, entrapment efficiency, ESR, NMR, X-ray diffraction, and biological effects. It is demonstrated that, contrary to what has been assumed, classical MLV exclude solutes during their formation and, thus, are under a state of osmotic compression. By contrast, the SPLV process produces liposomes that are not compressed. The effects of osmotic compression are discussed. It is suggested that the state of osmotic stress is an important variable that distinguishes various types of liposomes and that has significant physical and biological consequences.