CdTe QDs-based prostate-specific antigen probe for human prostate cancer cell imaging
CdTe QDs-based prostate-specific antigen probe for human prostate cancer cell imaging
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DOI：
10.1016/j.jlumin.2009.03.017
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发表时间：
2009-09-01
影响因子：
3.6
通讯作者：
Xu, Shukun
中科院分区：
文献类型：
--
作者：
Dong, Wei;Guo, Li;Xu, Shukun
在水溶液中直接制备了L-谷胱甘肽（GSH）稳定的CdTe量子点。将所制备的量子点连接到前列腺特异性抗原（PSA）用于直接标记，并连接到免疫球蛋白G（IgG）用于间接标记固定的前列腺癌细胞。结果表明，量子点探针是一种理想的荧光标记物，具有良好的光谱特性和光稳定性，比有机染料更适合于目标检测。间接标记法的特异性明显优于直接标记法。此外，所制备的CdTe量子点在培养3天后没有显示出可检测到的对细胞生长的影响，这表明L-谷胱甘肽封端的CdTe几乎没有细胞毒性。(C)2009 Elsevier B. V.保留所有权利。
L-glutathione (GSH) stabilized CdTe quantum dots (QDs) were directly prepared in aqueous solution. The as-prepared QDs were linked to prostate-specific antigen (PSA) for the direct labeling and linked to immunoglobulin G (IgG) for the indirect labeling of fixed prostate cancer cells. The results indicated that QD-based probes were ideal fluorescent markers with excellent spectral properties and photostability and much better than organic dyes making them very suitable in target detection. Meanwhile, the indirect labeling showed much better specificity than the direct labeling. Furthermore, the prepared CdTe QDs did not show detectable effect on cell growth after having cultured for three days, which suggested that the L-glutathione capped CdTe had scarcely cytotoxicity. (C) 2009 Elsevier B.V. All rights reserved.