Genetic variability within theAspergillus viridinutans species
Genetic variability within theAspergillus viridinutans species
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绿色曲霉物种内的遗传变异
DOI：
10.1007/bf02817615
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发表时间：
2008
影响因子：
2.6
通讯作者：
Z. Kozakiewicz
中科院分区：
文献类型：
--
作者：
J. Varga;B. Tóth;K. Rigó;F. Debets;Z. Kozakiewicz
采用形态学观察、碳源利用试验、线粒体和核DNA的限制性内切酶分析以及β-tubulin基因部分序列分析等技术，研究了该种的种内变异性。虽然分离株之间的遗传距离高于分离株之间的遗传距离，例如，A. fumigatus和Neuratoryafischeri，莫斯塔. viridinutansisolates和N.光环和N.根据序列数据发现Udagawaestreins属于一个单一的簇。菌株FRR 1266，其早先被分类为高度分歧的A。发现熏蒸剂属于A.绿虫种产赭曲霉毒素A的A.绿核壳菌IMI 306 135与无性分离株的亲缘关系最近。后两个菌株与A.烟曲霉N. fischerithan到任何。viridinutans菌株，并可能代表一个新种。基于碳源利用数据和线粒体和核DNA的限制性内切酶分析的结果的菌株的树状图支持的序列数据的基础上观察到的进化关系的大部分。结果表明，在烟曲霉组中，分生孢子头的有无并不是一个明确的形态学特征。
The intraspecific variability of theAspergillus viridinutansspecies was examined using various techniques including morphological examinations, carbon-source-utilization tests, restriction-enzyme analysis of the mitochondrial and nuclear DNA, and sequence analysis of part of the β-tubulin gene. Although the genetic distances between the isolates were higher than between,e.g., A. fumigatusandNeosartorya fischeri, mostA. viridinutansisolates, together withN. aureolaandN. udagawaestrains, were found to belong to a single cluster based on sequence data. Strain FRR 1266, which was earlier classified as a highly divergentA. fumigatusisolate, was found to belong to theA. viridinutansspecies. The ochratoxin A producingA. viridinutansstrain IMI 306 135 was most closely related to an asexual isolate. These two latter strains were more closely related toA. fumigatusandN. fischerithan to anyA. viridinutansstrains, and possibly represent a new species in theAspergillussectionFumigati. The dendrogram based on carbon source utilization data and results of restriction analysis of the mitochondrial and nuclear DNA of the strains supported most of the evolutionary relationships observed on the basis of sequence data. The results indicate that the presence or absence of nodding conidial heads is not an unequivocal morphological character for the identification of species within theAspergillussectionFumigati.