Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.
Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.
复制标题
从白色念珠菌中纯化肌动蛋白并与念珠菌 48,000-Mr 蛋白进行比较。
DOI：
10.1128/iai.55.9.2324-2326.1987
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发表时间：
1987
影响因子：
3.1
通讯作者：
Buckley,HR
中科院分区：
文献类型：
--
作者：
Fiss,E;Buckley,HR
采用DNase-Sepharose亲和层析法从白色念珠菌细胞中纯化肌动蛋白，并用鸡肌肌动蛋白单克隆抗体进行免疫印迹识别。侵袭性念珠菌病患者血清识别的白色念珠菌48千道尔顿蛋白与纯化的白色念珠菌肌动蛋白不相同。
Actin was purified from Candida albicans cells by affinity chromatography by DNase-Sepharose and was recognized by immunoblotting with monoclonal antibody directed against chick muscle actin. The C. albicans 48-kilodalton protein recognized by sera from patients with invasive candidiasis was shown by DEAE chromatography and immunoblotting not to be identical with the purified C. albicans actin.