Key determinants for morphine withdrawal conditioned context-induced increase in Arc expression in anterior cingulate cortex and withdrawal memory retrieval
Key determinants for morphine withdrawal conditioned context-induced increase in Arc expression in anterior cingulate cortex and withdrawal memory retrieval
复制标题
DOI：
10.1016/j.expneurol.2018.10.009
复制
发表时间：
2019-01-01
影响因子：
5.3
通讯作者：
Zheng, Ping
中科院分区：
文献类型：
--
作者：
Chen, Ming;Shao, Da;Zheng, Ping
成瘾记忆在远期以时间依赖的方式向新皮质转移，促进长期成瘾记忆的储存，在药物复吸中起着重要作用。然而，吗啡戒断条件作用后扣带回前部的活性和神经可塑性如何变化，以及是什么因素决定了这种变化仍有待确定。本文采用免疫组织化学和单细胞显微注射技术，结合行为学分析，发现：(1)条件性戒断条件刺激可增加吗啡戒断小鼠ACC中c-Fos和Arc的表达，但不影响Arc的表达：(2)条件性条件刺激对吗啡戒断小鼠ACC中c-Fos和Arc的表达无明显影响，(3)条件性戒断后第14天，条件性条件刺激可增加吗啡戒断小鼠ACC中c-Fos和Arc的表达；(4)抑制ACC树突棘或海马CA3区向ACC的投射神经元可减弱条件性语境诱导的ACC内Arc表达的增加，并使条件化后第14天的戒断记忆恢复完全消失。这些结果表明，在条件化处理后第14天，ACC的树突和从海马CA3到ACC的投射神经元可能表现出神经可塑性的改变，这是条件性语境诱导的ACC内Arc表达增加和条件化后第14天的戒断记忆提取的关键决定因素。
Addiction memory is subjected to time-dependent shifts toward neocortex in remote period to promote long-term addiction memory storage, which plays an important role in drug relapse. However, how the activity and neuroplasticity of the anterior cingulate cortex (ACC) change after contextual morphine withdrawal conditioning and what factors determine this change remain to be determined. In this paper, using immunohistochemical and single-cell microinjection techniques, combining behavioral assay, we found that (1) contextual withdrawal conditioning increases the expression of c-Fos, but not Arc, in the ACC in morphine withdrawal mice; (2) at the first day after conditioning, conditioned context has no influence on the expression of c-Fos and Arc in the ACC in morphine withdrawal mice; (3) at the 14th day after conditioning, conditioned context increases the expression of both c-Fos and Arc in the ACC in morphine withdrawal mice; (4) the inhibition of dendritic spines of the ACC or projection neurons from the CA3 of the hippocampus to the ACC attenuates the conditioned context-induced increase of Arc expression in the ACC and abolishes the retrieval of withdrawal memory at the 14th day after conditioning. These results suggest that the ACC may exhibit a change in neuroplasticity at the 14th day after conditioning, and the dendritic spines of the ACC and the projection neurons from the CA3 of the hippocampus to the ACC are key determinants for conditioned context induced increase in Arc expression in the ACC and the retrieval of withdrawal memory at the 14th day after conditioning.