Phenotypic characterization and genetic mapping of a new gene required for male and female gametophyte development in rice
Phenotypic characterization and genetic mapping of a new gene required for male and female gametophyte development in rice
复制标题
水稻雄性和雌性配子体发育所需的新基因的表型特征和遗传图谱
DOI：
10.1007/s11032-010-9520-3
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Ding, Lei;Li, Shuang Cheng;Li, Ping
配子体发育是开花植物的重要发育过程。种子是收获的主要器官，因此小穗肥力对水稻产量尤为重要。本文报道了一种新的水稻雌雄不育突变体mfs1的鉴定和遗传定位，该突变体是由粳稻品种中华9号转基因系产生的。花药横切面显示，这些植物的小孢子在减数分裂瘦素期异常退化，而绒毡层未退化，仍然存在；因此，突变体花药室中没有形成功能性花粉粒。此外，大多数突变胚囊未分化或分化不完全，导致无法形成功能性胚囊。对3个f2群体的遗传分析表明，突变表型受单隐性基因控制。通过连锁分析证实突变表型不是由于外源T-DNA插入。利用定位克隆技术对突变基因进行定位，最终将其定位到水稻2号染色体上简单序列重复标记RM12585和D051之间99kb的物理间隔上。序列分析表明，该区域共有11个水稻候选基因，包括3个编码的推测蛋白和8个编码的已知蛋白，如具有WRKY和锌指结构域的转录因子、一个泛素融合降解蛋白、伴侣蛋白clpb1、一个双组分响应调节因子、ZOS2-04-C2H2锌指蛋白、一个T1家族肽酶和一个蛋白激酶。
Gametophyte development is an important process in flowering plants. Seeds are the primary organs for harvesting, and therefore spikelet fertility is especially vital for rice yield. Here, we report the characterization and genetic mapping of a novel rice male and female sterile mutant,mfs1, which was generated from a transgenic line ofjaponicarice variety Zhonghua 9. Anther transverse sections indicate that the microspores in these plants were abnormally degenerated at the meiotic leptotene stage, while the tapetum layer was not degenerated and remained present; therefore, no functional pollen grains formed in the mutant anther locules. In addition, the majority of mutant embryo sacs did not undergo differentiation or the differentiation was incomplete, resulting in the absence of functional embryo sac formation. Genetic analysis of three F2populations indicated that the mutant phenotype was controlled by a single recessive gene. The mutant phenotype was confirmed by linkage analysis to be not due to a foreign T-DNA insertion. A map-based cloning strategy was employed to map the mutant gene, and, finally, themfs1locus was mapped to a 99-kb physical interval between simple sequence repeat markers RM12585 and D051 on rice chromosome 2. Sequence analysis indicates that 11 rice candidate genes are located in this region, including three encoded putative proteins and eight encoded known proteins, such as transcription factors having WRKY and zinc finger domains, a ubiquitin fusion degradation protein, chaperone protein clpB 1, a two-component response regulator, the ZOS2-04-C2H2 zinc finger protein, a peptidase of the T1 family and a protein kinase.