THE EFFECTS OF GLUCOSINOLATES AND THEIR HYDROLYSIS PRODUCTS ON MICROBIAL-GROWTH
THE EFFECTS OF GLUCOSINOLATES AND THEIR HYDROLYSIS PRODUCTS ON MICROBIAL-GROWTH
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DOI：
10.1111/j.1365-2672.1995.tb00931.x
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发表时间：
1995-08-01
期刊：
影响因子：
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通讯作者：
EDWARDS, C
中科院分区：
文献类型：
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作者：
BRABBAN, AD;EDWARDS, C
Rapemeal含有潜在毒性化合物，如芥子油苷，被评估为微生物生长的基质。硫代葡萄糖苷及其降解产物对一系列重要的工业微生物物种的影响进行了测试。Sinigrin（2-丙烯基芥子油苷）被认为是相对无害的所有测试的生物体，但其水解产生异硫氰酸酯，硫氰酸酯和腈导致生长抑制。发现水解前的初始抑制性黑芥子苷浓度是物种依赖性的，枯草芽孢杆菌最具抗性（80 μ g ml（-1）），酿酒酵母最敏感（40 μ g ml（-1））。三个革兰氏阳性微生物测试被认为是更耐水解产物比其他微生物。用异硫氰酸苯酯观察到类似的结果，发现其抑制是抑制剂和细胞浓度依赖性的。在含有芥子苷的液体培养基中，大肠杆菌在活跃生长期间添加硫代葡萄糖苷葡萄糖水解酶减少了活细胞的数量。在油菜籽培养基中也观察到类似的效果，其中生长抑制依赖于油菜籽中的硫代葡萄糖苷含量。
Rapemeal, which contains potentially toxic compounds such as glucosinolates, was assessed as a substrate for the growth of micro-organisms. The effects of glucosinolates and their degradation products were tested on a range of industrially important microbial species. Sinigrin (2-propenyl glucosinolate) was found to be relatively innocuous to all of the organisms tested but its hydrolysis to yield isothiocyanates, thiocyanates and nitriles resulted in inhibition of growth. The initial inhibitory sinigrin concentration before its hydrolysis was found to be species-dependent with Bacillus subtilis being the most resistant (80 mu g ml(-1)) and Saccharomyces cerevisiae (40 mu g ml(-1)) the most sensitive. Three Gram-positive organisms tested were found to be more resistant to hydrolysis products than other micro-organisms. Similar results were observed with phenylisothiocyanate for which inhibition was found to be inhibitor and cell concentration-dependent. Addition of thioglucoside glucohydrolase during active growth of Escherichia coli in a sinigrin-containing liquid medium reduced the number of viable cells. Similar effects were also observed in rapemeal media in which growth inhibition was dependent on the glucosinolate content of the rapemeal.