Sequence and organization of 5S RNA genes from the eukaryotic protist Acanthamoeba castellanii.
Sequence and organization of 5S RNA genes from the eukaryotic protist Acanthamoeba castellanii.
复制标题
真核原生生物卡氏棘阿米巴 5S RNA 基因的序列和组织。
DOI：
10.1016/0378-1119(91)90239-8
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发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Paule,MR
中科院分区：
文献类型：
--
作者：
Zwick,MG;Wiggs,M;Paule,MR
从卡氏阿米巴中克隆了一个5S RNA基因组克隆，并测定了其编码区和侧翼DNA的序列。该克隆编码的RNA序列与该生物体的5S RNA序列相匹配。该基因的5′侧翼区与其他真核生物的5 S RNA基因具有序列相似性，这些基因的转录活性需要上游区域或受上游区域的影响很大。紧邻的3′-侧翼区有一个终止序列，与所有由RNA聚合酶III转录的基因中发现的终止序列相似。A. Castellanii是分散的，这是非常不寻常的，因为大多数真核生物含有串联重复序列中聚集的5S基因。每个基因组中可能有多达480个编码5S RNA的基因。castellaniicell。
A 5S RNA genomic clone has been isolated fromAcanthamoeba castellaniiand the sequence of the coding region plus flanking DNA was determined. This clone encodes an RNA whose sequence matches that of 5 S RNA from this organism. There is sequence similarity in the 5′-flanking region to other eukaryotic 5 S RNA genes which require or are greatly affected by upstream regions for transcriptional activity. The immediate 3′-flanking region has a termination sequence similar to that found in all genes that are transcribed by RNA polymerase III. The 5S RNA genes ofA. castellaniiare dispersed, which is highly unusual, since the majority of eukaryotic organisms contain 5S genes clustered in tandem repeats. There may be up to 480 genes encoding 5 S RNA in eachA. castellaniicell.