Solid-phase nuclear magnetic resonance immunoassay for the prostate-specific antigen by using protein-coated magnetic nanoparticles
Solid-phase nuclear magnetic resonance immunoassay for the prostate-specific antigen by using protein-coated magnetic nanoparticles
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DOI：
10.1007/s00604-019-3925-4
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发表时间：
2019-12-01
影响因子：
5.7
通讯作者：
Rayev, Mikhail
中科院分区：
文献类型：
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作者：
Khramtsov, Pavel;Kropaneva, Maria;Rayev, Mikhail
提出了一种基于固相核磁共振的前列腺特异性抗原(PSA)的夹心免疫分析方法。用牛血清白蛋白对碳包裹的铁纳米颗粒进行功能化，偶联到单抗上，作为磁性标记物。将具有8微米孔的硝酸纤维素膜涂上捕获抗体，然后与血清样本和纳米结合物悬浮液孵育。测试试条被放置在便携式自制核磁共振弛豫仪中。磁性纳米粒子附着在硝化棉上，缩短了膜孔内水质子的T2驰豫时间。因此，T2与样本中抗原(PSA)的浓度成反比。该方法可在4h内完成，检出限为0.44ngmL(-1)。激肽释放酶2、人绒毛膜促性腺激素和甲胎蛋白不会产生干扰。
A solid phase NMR-based sandwich immunoassay for the prostate-specific antigen (PSA) is presented. Carbon-encapsulated iron nanoparticles were functionalized with bovine serum albumin, coupled to monoclonal antibodies, and then used as magnetic labels. A nitrocellulose membrane with 8-mu m pores was coated with capture antibodies and subsequently incubated with a serum sample and a suspension of the nanoconjugate. Test strips were placed in a portable homemade NMR relaxometer. Magnetic nanoparticles attached to nitrocellulose decrease the T2 relaxation time of the water protons located inside the pores of the membrane. Thus, T2 is inversely proportional to the concentration of the antigen (PSA) in the sample. The assay can be performed within 4 h. The detection limit is 0.44 ng mL(-1). Kallikrein 2, human chorionic gonadotropin, and alpha-fetoprotein do not interfere.