Anthelmintic activity of steroidal saponins from Paris polyphylla
Anthelmintic activity of steroidal saponins from Paris polyphylla
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DOI：
10.1016/j.phymed.2010.04.012
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发表时间：
2010-12-01
期刊：
影响因子：
7.9
通讯作者：
Liu, X. -L.
中科院分区：
文献类型：
--
作者：
Wang, G. -X.;Han, J.;Liu, X. -L.
对巴黎根茎粗提物和纯品进行了驱虫活性研究。甲醇提取物对中间指环虫具有较好的驱虫活性（EC（50）值为18.06 mg l（-1））。基于这些发现，甲醇提取物在硅胶柱色谱上在生物测定引导的分级分离中分级分离，得到两种已知的显示出有效活性的甾体皂苷，薯蓣皂苷（1）和polyphyllin 0（2）。薯蓣皂苷和多叶薯蓣皂苷D均表现出明显的抗真菌活性。结果表明，对中间体的EC（50）值分别为0.44和0.70 mg·l（-1），明显高于阳性对照甲苯咪唑（EC（50）值为1.25 mg·l（-1））。薯蓣皂苷和叶多甙D对金鱼的急性毒性（LC（50））分别为1.37和1.08 mg l（-1）。这些结果表明，P. polyphylla提取物和分离的化合物是潜在的天然药剂，用于控制指环虫感染。本文首次报道了多叶原甲的体内驱虫研究。Crown版权所有（C）2010由Elsevier GmbH出版。All rights reserved.
The present study was undertaken to investigate the anthelmintic activity of crude extracts and pure compounds from the rhizomes of Paris polyphylla. The methanol extract showed a promising anthelmintic activity against Dactylogyrus intermedius (EC(50) value = 18.06 mg l(-1)). Based on these finding, the methanol extract was fractionated on silica gel column chromatography in a bioassay-guided fractionation affording two known steroidal saponins showing potent activity, dioscin (1) and polyphyllin 0(2). Both dioscin and polyphyllin D exhibited significant activity against D. intermedius with EC(50) values of 0.44 and 0.70 mg l(-1), respectively, which were more effective than the positive control, mebendazole(EC(50) value = 1.25 mg l(-1)). The acute toxicities (LC(50)) of dioscin and polyphyllin D for goldfish were 1.37 and 1.08 mg l(-1), respectively. These results indicated that P. polyphylla extract and the isolated compounds are potential natural agents for the control of Dactylogyrus infestation. This is the first report on in vivo anthelmintic investigation for P. polyphylla. Crown Copyright (C) 2010 Publihed by Elsevier GmbH. All rights reserved.