POLYADENYLATION OF A HUMAN MITOCHONDRIAL RIBOSOMAL-RNA TRANSCRIPT DETECTED BY MOLECULAR-CLONING
POLYADENYLATION OF A HUMAN MITOCHONDRIAL RIBOSOMAL-RNA TRANSCRIPT DETECTED BY MOLECULAR-CLONING
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DOI：
10.1016/0378-1119(85)90009-5
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发表时间：
1985-01-01
期刊：
影响因子：
3.5
通讯作者：
BENZ, EJ
中科院分区：
文献类型：
--
作者：
BASERGA, SJ;LINNENBACH, AJ;BENZ, EJ
通过分子克隆鉴定了人白血病细胞系K562中的多腺苷酸化RNA转录物，其与编码线粒体16 S rRNA（mt 16 S rRNA）的基因的一部分互补。转录本的克隆部分对应于人类mt基因组的位置2191-2395。该克隆代表来自H链的RNA转录物的互补DNA拷贝，并在其3“-末端携带额外的21个残基长的poly（A）尾。这些数据为某些mt 16 S rRNA转录物的多聚腺苷酸化提供了直接证据。
A polyadenylated RNA transcript in the human leukemia cell line, K562, was identified by molecular cloning, which is complementary to a portion of the gene-encoding mitochondrial 16S rRNA (mt 16S rRNA). The cloned portion of the transcript corresponds to positions 2191-2395 of the human mt genome. The clone represents a complementary DNA copy of an RNA transcript from the H strand and carries an additional poly(A) tail 21 residues long at its 3''-end. The data provide direct evidence for polyadenylation of some mt 16S rRNA transcripts.