Identification and molecular analysis of a lneRNA-HOTAIR transcript from secondary hair follicle of cashmere goat reveal integrated regulatory network with the expression regulated potentially by its promoter methylation
Identification and molecular analysis of a lneRNA-HOTAIR transcript from secondary hair follicle of cashmere goat reveal integrated regulatory network with the expression regulated potentially by its promoter methylation
复制标题
绒山羊次级毛囊 lncRNA-HOTAIR 转录物的鉴定和分子分析揭示了整合的调控网络，其表达可能受其启动子甲基化调节
DOI：
10.1016/j.gene.2018.11.084
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发表时间：
2019-03-10
期刊：
影响因子：
3.5
通讯作者：
Bai, Wen L.
中科院分区：
文献类型：
--
作者：
Jiao, Qian;Yin, Rong H.;Bai, Wen L.
HOTAIR转录本是从HOXC基因簇内的反义链转录的，并且被认为在毛囊重建期间在调节毛乳头细胞的诱导能力中起作用。在本研究中，我们首次从绒山羊次级毛囊中分离并鉴定了lncRNA-HOTAIR转录本。分析了HOTAIR基因启动子在绒山羊生长期和休止期次级毛囊中的转录模式和甲基化水平。核苷酸组成分析表明，绒山羊lncRNA-HOTAIR转录本中腺嘌呤（A）和胸腺嘧啶（T）的含量高于鸟嘌呤（G）和胞嘧啶（C），其中AG核苷酸对的频率分布最高（8.06%）。调控网络分析表明，绒山羊lncRNA-HOTAIR与其潜在靶分子miRNAs、mRNAs和蛋白质之间存在直接或间接的复杂调控关系。lncRNA-HOTAIR基因在绒山羊次级毛囊生长期和休止期均有转录，且在生长期的表达量显著高于休止期，提示lncRNA-HOTAIR基因可能参与了次级毛囊的重建和绒纤维的形成与生长。结合HOTAIR基因启动子甲基化分析，我们的研究结果表明，HOTAIR基因启动子甲基化可能参与了绒山羊次级毛囊HOTAIR基因的转录抑制。
The HOTAIR transcript is transcribed from the antisense strand within the HOXC gene cluster, and it is thought to play a role in regulating the inductive capacity of dermal papilla cells during the reconstruction of hair follicle. In the current investigation, we firstly isolated and characterized a lncRNA-HOTAIR transcript from the secondary hair follicle of cashmere goat. Also, we analyzed its transcriptional pattern and methylation level of HOTAIR gene promoter in secondary hair follicle of cashmere goat during anagen and telogen stages. Nucleotide composition analysis indicated that the contents of Adenine (A) and Thymine (T) are higher than that of Guanine (G) and Cytosine (C) in lncRNA-HOTAIR transcript of cashmere goat with the highest frequency distribution of AG nucleotide pair (8.06%). The regulatory network analysis showed a directly or indirectly complex regulatory relationships between lncRNA-HOTAIR of cashmere goat and its potential target molecules: miRNAs, mRNAs and proteins. Also, we showed that lncRNA-HOTAIR was properly transcribed at both anagen and telogen stages of secondary hair follicle of cashmere goat with the anagen being significantly higher than telogen in its expression, which suggest that lncRNA-HOTAIR transcript might be involved in the reconstruction of secondary hair follicle with the formation and growth of cashmere fiber. Taken together with methylation analysis of HOTAIR gene promoter, our data suggest that the promoter methylation of HOTAIR gene most likely is involved in its transcriptional suppression in secondary hair follicle of cashmere goat.