Thyroid parafollicular cells. An accessible model for the study of serotonergic neurons.
Thyroid parafollicular cells. An accessible model for the study of serotonergic neurons.
复制标题
甲状腺滤泡旁细胞。
DOI：
10.1007/bf02740626
复制
发表时间：
1996
影响因子：
5.1
通讯作者：
Durham,PL
中科院分区：
文献类型：
--
作者：
Russo,AF;Clark,MS;Durham,PL
血清素能神经元在调节多种行为和体内平衡过程中发挥关键作用。然而，在分子水平上研究这些神经元的良好模型系统缺乏。在这篇综述中，我们将提供证据，证明来自一个意想不到的来源的细胞系，甲状腺滤泡旁细胞（PF）（也称为C细胞），符合作为研究血清素能神经元模型的标准。与其他细胞系相比，PF细胞系的优势在于亲代PF细胞具有血清素能特性和神经电位，这与它们的神经嵴起源一致。此外，PF细胞和PF细胞系能够表达5-羟色胺能神经元的基本特性，包括：(1)5-羟色胺（5-HT）由色氨酸羟化酶（TPH）生物合成，(2)5-HT的囊泡储存和调节释放，(3)5-HT自身受体的表达，(4)5-HT转运体的表达。在这篇综述中，我们将主要关注大鼠CA77 PF细胞系和亲本大鼠PF细胞的血清素能和神经元特性。将描述CA77细胞用于分子分析的适用性。首先，将讨论它们在糖皮质激素调控TPH基因研究中的应用。其次，将讨论降钙素/降钙素基因相关肽（CT/CGRP）基因的控制，特别强调5 -羟色胺能药物在偏头痛治疗中的应用。这些例子突出了甲状腺PF细胞系作为研究血清素生物合成和生理作用控制系统的多功能性。
Serotonergic neurons play key roles in modulating a wide variety of behavioral and homeostatic processes. However, there is a paucity of good model systems to study these neurons at a molecular level. In this review we will present evidence that cell lines derived from an unexpected source, thyroid parafollicular cells (PF) (also called C cells), fit the criteria for use as models for the study of serotonergic neurons. A strength of PF cell lines over other cell lines is that the parental PF cells have serotonergic properties and a neuronal potential that is consistent with their neural crest origin. Futhermore, PF cells and PF cell lines are capable of expressing the fundamental properties of serotonergic neurons, including: (1) serotonin (5-HT) biosynthesis by tryptophan hydroxylase (TPH), (2) vesicular 5-HT storage and regulated release, (3) expression of a 5-HT autoreceptor, and (4) expression of the 5-HT transporter. In this review, we will focus primarily on the serotonergic and neuronal properties of the rat CA77 PF cell line and the parental rat PF cells. The applicability of CA77 cells for molecular analyses will be described. First, their use for studies on the glucocorticoid regulation of the TPH gene will be discussed. Second, control of the calcitonin/calcitonin gene-related peptide (CT/CGRP) gene will be discussed, with particular emphasis on the application of serotonergic drugs in treating migraine headaches. These examples highlight the versatility of thyroid PF cell lines as a system for studying the control of both serotonin biosynthesis and physiological actions.