Arabidopsis F-BOX STRESS INDUCED 4 is required to repress excessive divisions in stomatal development
Arabidopsis F-BOX STRESS INDUCED 4 is required to repress excessive divisions in stomatal development
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拟南芥 FâBOX STRESS INDUCED 4 是抑制气孔发育过度分裂所必需的
DOI：
10.1111/jipb.13193
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发表时间：
2022
影响因子：
11.4
通讯作者：
Le Jie
中科院分区：
文献类型：
--
作者：
Li Yi;Xue Shan;He Qixiumei;Wang Junxue;Zhu Lingling;Zou Junjie;Zhang Jie;Zuo Chaoran;Fan Zhibin;Yue Junling;Zhang Chunxia;Yang Kezhen;Le Jie
在气孔发育的末期，R2 R3-MYB转录因子FOUR LIPS（FLP/MYB 124）和MYB 88通过抑制多个细胞周期基因的转录水平来限制保卫母细胞的分裂。在具有弱等位基因flp-1的拟南芥中，额外的保卫母细胞分裂导致两个气孔直接接触。在这里，我们发现了一个乙基甲烷磺酸诱变突变体flp-1 xs 01 c，它比单独的flp-1表现出更严重的缺陷，产生巨大的肿瘤样细胞簇。XS 01 C基因编码F-BOX STRESS-INDUCED 4（FBS 4），在表皮气孔前体细胞中优先表达。过表达FBS 4挽救了flp-1 xs 01 c和flp-1突变体缺陷的气孔表型。FBS 4的F-box结构域中保守残基（脯氨酸166）的缺失或取代分别消除或减少了其与拟南芥Skp 1-Like 1（ASK 1）（Skp 1/Cullin/F-box E3泛素连接酶复合物的核心亚基）的相互作用。此外，FBS 4蛋白与CYCA 2;3物理相互作用，并通过泛素-26 S蛋白酶体途径诱导其降解。因此，除了已知的转录途径之外，气孔发育中的末端对称分裂在翻译后水平上得到确保，例如通过气孔谱系F-box蛋白FBS 4识别的靶蛋白的泛素化。
During the terminal stage of stomatal development, the R2R3-MYB transcription factors FOUR LIPS (FLP/MYB124) and MYB88 limit guard mother cell division by repressing the transcript levels of multiple cell-cycle genes. In Arabidopsis thaliana possessing the weak allele flp-1, an extra guard mother cell division results in two stomata having direct contact. Here, we identified an ethylmethane sulfonatemutagenized mutant, flp-1 xs01c, which exhibited more severe defects than flp-1 alone, producing giant tumor-like cell clusters. XS01C, encoding F-BOX STRESS-INDUCED 4 (FBS4), is preferentially expressed in epidermal stomatal precursor cells. Overexpressing FBS4 rescued the defective stomatal phenotypes of flp-1 xs01c and flp-1 mutants. The deletion or substitution of a conserved residue (Proline166) within the F-box domain of FBS4 abolished or reduced, respectively, its interaction with Arabidopsis Skp1-Like1 (ASK1), the core subunit of the Skp1/Cullin/F-box E3 ubiquitin ligase complex. Furthermore, the FBS4 protein physically interacted with CYCA2;3 and induced its degradation through the ubiquitin-26S proteasome pathway. Thus, in addition to the known transcriptional pathway, the terminal symmetric division in stomatal development is ensured at the post-translational level, such as through the ubiquitination of target proteins recognized by the stomatal lineage F-box protein FBS4.