Reliable addition of reagents into microfluidic droplets
Reliable addition of reagents into microfluidic droplets
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DOI：
10.1007/s10404-009-0531-5
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发表时间：
2010-03
影响因子：
2.8
通讯作者：
Jayaprakash Sivasamy;Y. C. Chim;T. Wong;N. Nguyen;L. Yobas
中科院分区：
文献类型：
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作者：
Jayaprakash Sivasamy;Y. C. Chim;T. Wong;N. Nguyen;L. Yobas
本文报道了一种设计，可靠地添加到液滴的试剂，利用流体流动的物理在T形接头的微通道。在T形接头之后的扩展部分增强了液流与液滴的合并，消除了诸如额外液滴形成和长混合时间的缺点。扩展部分减少了T形接头处的压力积累，并最大限度地减少了形成额外液滴的趋势;在T形接头形成的液滴界面上产生低拉普拉斯压力跳变，这降低了在合并过程中形成额外液滴的可能性;如果由于合并前液滴破裂而存在额外液滴，则为液滴聚结提供空间。在这种设计中，在合并之后，反应物在液滴内呈轴向排列，这导致更快的混合。对于去离子水（Q DI）和荧光（Q fluo）流，在25至250 μl/h的宽流速范围内，可将试剂可靠地添加到液滴中。
This article reports a design that reliably adds reagents into droplets by exploiting the physics of fluid flow at a T-junction in the microchannel. An expanded section right after the T-junction enhances merging of a stream with a droplet, eliminates the drawbacks such as extra droplet formation and long mixing time. The expanded section reduces the pressure buildup at the T-junction and minimizes the tendency to form extra droplets; plays the role in creating low Laplace pressure jump across the interface of the droplet forming from the T-junction which reduces the probability of forming extra droplet in the merging process; provides space for droplet coalescence if there is an extra droplet due to droplet break-up before merging. In this design, after merging, the reactants are in axial arrangement inside the droplets which lead to faster mixing. Reliable addition of reagent to the droplets happens for the combination of flow rates in a broad range from 25 to 250 μl/h, for both DI water (Q DI) and fluorescent (Q fluo) streams.