Establishment of an allergic rhinitis model in mice for the evaluation of nasal symptoms
Establishment of an allergic rhinitis model in mice for the evaluation of nasal symptoms
复制标题
DOI：
10.1016/j.lfs.2006.12.038
复制
发表时间：
2007-03-20
期刊：
影响因子：
6.1
通讯作者：
Kino, Kohsuke
中科院分区：
文献类型：
--
作者：
Tsunematsu, Masako;Yamaji, Taketo;Kino, Kohsuke
我们研究的目的是建立一种新的小鼠过敏性鼻炎模型，引发打喷嚏、嗜酸性粒细胞浸润鼻粘膜以及抗原特异性 IgE 产生等症状。 Cry j 1（日本柳杉花粉过敏原）中的主要人类 T 细胞表位之一，也是 B10.S 小鼠中的主要鼠 T 细胞表位。因此我们尝试以Cry j 1为抗原建立B10.S小鼠过敏性鼻炎模型。我们用 Cry j 1/明矾皮下致敏 B10.S 小鼠 3 次，间隔 1 周。最终致敏后五周，我们从鼻内组胺预处理后的第二天起通过鼻内滴注 Cry j 1 来挑战小鼠。不久之后，我们数了喷嚏的次数。然后我们评估了鼻组织中嗜酸性粒细胞的浸润情况，并测量了抗原特异性 IgE 抗体的血清水平。此外，我们还证实了富马酸酮替芬和盐酸地塞米松对这些动物的影响。在 Cry j 1 致敏的 B10.S 小鼠中，鼻内组胺预处理和连续鼻内 Cry j 1 攻击 5 天后，喷嚏、鼻组织中的嗜酸性粒细胞过氧化物酶 (EPO) 活性和 Cry j 1 特异性 IgE 明显增加。酮替芬和地塞米松均抑制喷嚏的增加，地塞米松还抑制EPO活性和Cry j 1特异性IgE。因此，我们成功地在Cry j 1致敏的B10.S小鼠中建立了一种新的过敏性鼻炎模型，该模型表现出打喷嚏、嗜酸性粒细胞浸润鼻粘膜以及Cry j 1特异性IgE的产生。 (c) 2007 Elsevier Inc. 保留所有权利。
The purpose of our study was to establish a new model of allergic rhinitis in mice, eliciting symptoms such as sneezing, infiltration of eosinophils into the nasal mucosa, and antigen-specific IgE production. One of the major human T-cell epitopes in Cry j 1, an allergen of Japanese cedar pollen, is also a major murine T-cell epitope in B10.S mice. Thus we tried to establish an allergic rhinitis model in B10.S mice with Cry j 1 as the antigen. We sensitized B10.S mice subcutaneously with Cry j 1/alum three times at intervals of 1 week. Five weeks after the final sensitization, we challenged the mice by instilling Cry j 1 intranasally from the day after intranasal histamine pretreatment. Soon after, we counted the number of sneezes. We then evaluated the infiltration of eosinophils into the nasal tissues and also measured the serum levels of antigen-specific IgE antibody. In addition, we confirmed the effects of ketotifen fumarate and dexamethasone hydrochloride on these animals. In Cry j 1 sensitized B10.S mice, sneezes, eosinophil peroxidase (EPO) activity in nasal tissues, and Cry j 1-specific IgE clearly increased after intranasal histamine pretreatment and 5 days of continuous intranasal Cry j 1 challenge. Both ketotifen and dexamethasone inhibited the increase in sneezing, and dexamethasone also inhibited EPO activity and Cry j 1-specific IgE. Thus we succeeded in establishing a new model of allergic rhinitis in Cry j 1-sensitized B10.S mice, which exhibited sneezing, eosinophil infiltration into the nasal mucosa, and Cry j 1-specific IgE production. (c) 2007 Elsevier Inc. All rights reserved.