Archaic strains of methicillin-resistant Staphylococcus aureus:: Molecular and microbiological properties of isolates from the 1960s in Denmark
Archaic strains of methicillin-resistant Staphylococcus aureus:: Molecular and microbiological properties of isolates from the 1960s in Denmark
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DOI：
10.1089/mdr.2000.6.1
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发表时间：
2000-03-01
期刊：
影响因子：
--
通讯作者：
Westh, H
中科院分区：
文献类型：
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作者：
De Lencastre, H;Chung, M;Westh, H
20世纪60年代在丹麦分离的耐甲氧西林金黄色葡萄球菌（MRSA）是这些细菌历史上最早的样本之一。我们确定了46个这样的分离株的微生物和分子特性。它们表现出非常一致的特性，包括：（i）低甲氧西林MIC值（6 - 25 μ g/ml）;（ii）抗性的异质表达;（iii）存在单一的、常见的mecA多晶型物II;（iv）缺乏调节基因mec1;（v）经常缺乏Tn554;和（vi）常见的脉冲场凝胶电泳（PFGE）类型。这些特性，连同分离的时间顺序日期，以及从分布在丹麦的18家医院回收的菌株，表明它们代表了一个在时间上接近欧洲MRSA菌株进化起源的谱系。
Methicillin-resistant Staphylococcus aureus (MRSA) isolated in the 1960s in Denmark are among the historically earliest samples of these bacteria. We determined microbiological and molecular properties of 46 such isolates. They showed remarkably uniform properties, which included: (i) low methicillin MIC value (6-25 mu g/ml); (ii) heterogeneous expression of resistance; (iii) the presence of a single, common, mecA polymorph II; (iv) lack of the regulatory gene mec1; (v) frequent lack of Tn554; and (vi) a common pulsed-field gel electrophoresis (PFGE) type. These properties, together with the chronological dates of isolation, and recovery of the strains from 18 hospitals scattered over Denmark, suggest that they represent a lineage close in time to the evolutionary origin of European strains of MRSA.