Comparative analysis of seven mitochondrial genomes of Phymatostetha (Hemiptera: Cercopidae) and phylogenetic implications
Comparative analysis of seven mitochondrial genomes of Phymatostetha (Hemiptera: Cercopidae) and phylogenetic implications
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Phymatostetha（半翅目：鹿科）七个线粒体基因组的比较分析及其系统发育意义
DOI：
10.1016/j.ijbiomac.2018.12.174
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发表时间：
2019
影响因子：
8.2
通讯作者：
Liang Aiping
中科院分区：
文献类型：
--
作者：
Su Tianjuan;Liang Aiping
在这项研究中，我们提出了 Phymatostetha 的七个线粒体基因组（线粒体基因组）。每个线粒体基因组包含整套 37 个基因，其排列顺序与假定的昆虫祖先模式相同。 Phymatostethamitogenomes的核苷酸组成偏向A/T，rRNA和PCG12（即PCG的第一个和第二个密码子位置）分别表现出最高和最低的A+T含量。 PCG 的相对同义密码子使用也表明简并密码子倾向于使用更多的 A/T 而不是 G/C。除 trnS1 外，所有 tRNA 均表现出典型的三叶草结构。此外，在 trnS1 反密码子茎和 trnR 受体茎中检测到未配对的核苷酸。使用贝叶斯法和最大似然法分析基于 13 个 PCG、22tRNA 和 2 个 rRNA 的数据集的系统发育关系。我们的结果清楚地揭示了Phymatostetha种的系统地位，并有力地支持了该属的单系性，其中Phymatostetha semelei是其他Phymatostetha种的姐妹。事实证明，丝裂基因组是一种有效的分子标记，可以在低分类水平上充分解析系统发育。
In this study, we present seven mitochondrial genomes (mitogenomes) ofPhymatostetha. Each mitogenome contains the entire set of 37 genes, which arranged in the same order as the putative ancestral pattern of insects. The nucleotide composition ofPhymatostethamitogenomes is biased toward A/T, with rRNAs and PCG12 (i.e. the first and second codon positions of PCGs) exhibit the highest and lowest A + T content, respectively. Relative synonymous codon usage of PCGs also show that degenerate codons are biased to use more A/T than G/C. All tRNAs exhibit typical clover-leaf structure, with the exception oftrnS1. Additionally, unpaired nucleotides are detected intrnS1anticodon stem andtrnRacceptor stem. Phylogenetic relationships, based on the dataset of 13 PCGs, 22tRNAs, and two rRNAs, are analyzed using both the Bayesian and maximum likelihood methods. Our results clearly revealed the systematic status ofPhymatostethaspecies and robustly supported the monophyly of this genus, in whichPhymatostetha semeleis sister to otherPhymatostethaspecies. It was demonstrated that mitogenome was an effective molecular marker to adequately resolve phylogeny at low taxonomic levels.