gamma delta T cells do not play a major role in controlling infection in experimental cysticercosis.
gamma delta T cells do not play a major role in controlling infection in experimental cysticercosis.
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γ δ T 细胞在控制实验性囊尾蚴病感染方面不起主要作用。
DOI：
10.1017/s0031182099004771
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发表时间：
1999
期刊：
影响因子：
2.4
通讯作者：
Kuhn,RE
中科院分区：
文献类型：
--
作者：
Toenjes,SA;Spolski,RJ;Mooney,KA;Kuhn,RE
针对粗头带绦虫幼虫的保护性免疫已被证明依赖于T细胞;然而，在感染期间T细胞的特定亚群的作用尚不清楚。为了研究γδT细胞的可能作用，本研究研究了δ链敲除C57 BL/6（deltaKO）和野生型C57 BL/6小鼠中的幼虫感染。发现deltaKO小鼠和C57 BL/6小鼠对感染同样敏感，表明γδT细胞在保护性免疫中不起主要作用。测定了伴刀豆球蛋白A（ConA）刺激的来自感染的deltaKO小鼠和C57 BL/6小鼠的脾细胞的细胞因子产生。所有感染的小鼠表现出增加的IL-10产生，表明Th 1抑制功能。来自感染的deltaKO小鼠和C57 BL/6小鼠的细胞未显示IL-4产生的增加。与deltaKO小鼠相比，重度感染的C57 BL/6小鼠显示IFN-γ产生减少。这些观察结果表明，IL-10产生的增加与非保护性免疫应答最相关。为了在体外细胞因子产生和全身免疫应答之间进行比较，测定血清中的细胞因子水平。C57 BL/6小鼠和deltaKO小鼠在感染后52天显示IL-4和IFN-γ的血清水平增加。因此，这些小鼠的全身免疫应答是混合的Th 1/Th 2型应答，并且γδT细胞显然不负责这些细胞因子的全身性增加。
Protective immunity against larval Taenia crassiceps has been shown to rely on T cells; however, the roles of the specific subsets of T cells during infection are not known. To investigate a possible role for γδT cells, this study investigated larval infection in δ-chain knock-out C57BL/6 (deltaKO) and wild-type C57BL/6 mice. It was found that deltaKO mice and C57BL/6 mice were equally susceptible to infection suggesting γδT cells do not play a major role in protective immunity. Cytokine production by concanavalin A (ConA)-stimulated spleen cells from infected deltaKO mice and C57BL/6 mice were determined. All infected mice demonstrated an increased IL-10 production suggesting a Th1-inhibitory function. Cells from infected deltaKO mice and C57BL/6 mice did not show increases in IL-4 production. Heavily-infected C57BL/6 mice showed a decrease in IFN-γ production compared to deltaKO mice. These observations suggest that an increase in IL-10 production best correlates with a non-protective immune response. To make comparisons between in vitro cytokine production and systemic immune responses, cytokine levels in serum were determined. C57BL/6 mice and deltaKO mice showed increases in serum levels of IL-4 and IFN-γ at 52 days post-infection. The systemic immune response of these mice, therefore, is a mixed Th1/Th2-type response and γδT cells are apparently not responsible for the systemic increases in these cytokines.