An endoplasmic reticulum magnesium transporter is essential for pollen development in Arabidopsis
An endoplasmic reticulum magnesium transporter is essential for pollen development in Arabidopsis
复制标题
内质网镁转运蛋白对于拟南芥花粉发育至关重要
DOI：
10.1016/j.plantsci.2014.12.008
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发表时间：
2015-02-01
期刊：
影响因子：
5.2
通讯作者：
Li, Dongping
中科院分区：
文献类型：
--
作者：
Li, Jian;Huang, Yuan;Li, Dongping
镁是植物生长发育所必需的大量元素之一，参与光合作用和多种代谢过程。在细菌或酵母突变体系统中鉴定了AtMGT（Magnesium Transporter）基因的镁转运能力。在我们以前的研究中，发现AtMGT 5和AtMGT 9都是拟南芥花粉发育所必需的。在这里，我们报告的另一个AtMGT成员，AtMGT 4，这是本地化的内质网，是必不可少的花粉发育。AtMGT 4在二细胞花粉期至成熟花粉期的花粉粒中均有显著表达。该基因的T-DNA插入突变体mgt 4 -1表现花粉败育表型，因此在+/mgt 4 -1自交后代中不能获得纯合突变体。同时，AtMGT 4-RNAi转基因株系中近一半的花粉为不育，与+/mgt 4 -1突变体的表型一致。在mgt 4 -1背景下表达AtMGT 4的转基因植株可以恢复到野生型的花粉育性。总之，我们的研究结果表明，AtMGT 4在拟南芥中的破坏可能会导致花粉发育的缺陷。在+/mgt 4 -1中，可见的花粉败育发生在二细胞花粉期。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Magnesium is one of the essential macro-elements for plant growth and development, participated in photosynthesis and various metabolic processes. The Mg-transport abilities of the AtMGT (Magnesium Transporter) genes were identified in bacteria or yeast mutant system. In our previous studies, both the AtMGT5 and AtMGT9 were found essential for pollen development in Arabidopsis. Here we report another AtMGT member, AtMGT4, which was localized to the endoplasmic reticulum, was essential for pollen development as well. AtMGT4 expressed notably in pollen grains from bicellular pollen stage to mature pollen stage. A T-DNA insertional mutant of the gene, named mgt4-1, showed pollen abortive phenotype, thus we could not get any homozygous mutant from progenies of self-crossed +/mgt4-1 plants. Meanwhile, nearly half of pollens in AtMGT4-RNAi transgenic lines were sterile, consistent with the phenotype of +/mgt4-1 mutant. Transgenic plants expressing AtMGT4 in the mgt4-1 background could recover the pollen fertility to the wild type. Together, our findings demonstrated that the disruption of AtMGT4 in Arabidopsis could cause a defect of pollen development. The visible pollen abortion appeared at bicellular pollen stage in +/mgt4-1. (C) 2014 Elsevier Ireland Ltd. All rights reserved.