Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia.
Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia.
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DOI：
10.1007/s10156-008-0648-6
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
Acute Respiratory Diseases Study Group
中科院分区：
文献类型：
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作者：
Hamano-Hasegawa K;Morozumi M;Nakayama E;Chiba N;Murayama SY;Takayanagi R;Iwata S;Sunakawa K;Ubukata K;Acute Respiratory Diseases Study Group
我们开发了一种实时逆转录PCR（RT-PCR）方法来检测13种呼吸道病毒：流感病毒A和B；呼吸道合胞病毒 (RSV) A 和 B 亚型；副流感病毒 (PIV) 1、2 和 3；腺病毒；鼻病毒（RV）；肠道病毒；冠状病毒（OC43）；人类偏肺病毒（hMPV）；和人类博卡病毒（HBoV）。检测这些病毒的新方法与实时 PCR 同时应用，检测了 1700 名社区获得性肺炎 (CAP) 儿科患者的临床样本中的六种细菌病原体。所有患者中，32.5%疑似单一细菌感染； 1.9%，多种细菌感染； 15.2%，细菌和病毒合并感染； 25.8%，单一病毒感染； 2.1%，多种病毒感染。其余22.6%的病因不明。疑似病原菌分布如下：肺炎链球菌占24.4%，肺炎支原体占14.8%，流感嗜血杆菌占11.3%，肺炎衣原体占1.4%。病毒分类如下：RV 占 14.5%，RSV 占 9.4%，hMPV 占 7.4%，PIV 占 7.2%，HBoV 占 2.9%。新方法将有助于提高诊断的准确性，并有助于适当使用抗菌药物。
We have developed a real-time reverse transcription-PCR (RT-PCR) method to detect 13 respiratory viruses: influenza virus A and B; respiratory syncytial virus (RSV) subgroup A and B; parainfluenza virus (PIV) 1, 2, and 3; adenovirus; rhinovirus (RV); enterovirus; coronavirus (OC43); human metapneumovirus (hMPV); and human bocavirus (HBoV). The new method for detection of these viruses was applied simultaneously with real-time PCR for the detection of six bacterial pathogens in clinical samples from 1700 pediatric patients with community-acquired pneumonia (CAP). Of all the patients, 32.5% were suspected to have single bacterial infections; 1.9%, multiple bacterial infections; 15.2%, coinfections of bacteria and viruses; 25.8%, single viral infections; and 2.1%, multiple viral infections. In the remaining 22.6%, the etiology was unknown. The breakdown of suspected causative pathogens was as follows: 24.4% were Streptococcus pneumoniae, 14.8% were Mycoplasma pneumoniae, 11.3% were Haemophilus influenzae, and 1.4% were Chlamydophila pneumoniae. The breakdown of viruses was as follows: 14.5% were RV, 9.4% were RSV, 7.4% were hMPV, 7.2% were PIV, and 2.9% were HBoV. The new method will contribute to advances in the accuracy of diagnosis and should also result in the appropriate use of antimicrobials.