THE REELER GENE-ASSOCIATED ANTIGEN ON CAJAL-RETZIUS NEURONS IS A CRUCIAL MOLECULE FOR LAMINAR ORGANIZATION OF CORTICAL-NEURONS
THE REELER GENE-ASSOCIATED ANTIGEN ON CAJAL-RETZIUS NEURONS IS A CRUCIAL MOLECULE FOR LAMINAR ORGANIZATION OF CORTICAL-NEURONS
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DOI：
10.1016/0896-6273(95)90329-1
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发表时间：
1995-05-01
期刊：
影响因子：
16.2
通讯作者：
MIKOSHIBA, K
中科院分区：
文献类型：
--
作者：
OGAWA, M;MIYATA, T;MIKOSHIBA, K
在神经学突变的小鼠卷取器中，新皮质的组织结构发育异常，本质上导致皮质层的相对位置颠倒。因此，卷轴突变提供了对皮质层形成的分子机制的洞察。我们已经产生了一种单抗(CR-50)，它探测野生型小鼠中存在的一种独特的等位基因抗原，但在卷轴突变小鼠中不存在。CR-50与Cajal-Retzius神经元发生特异性反应，Cajal-Retzius神经元是最早在新皮质分化的皮质神经元之一，但其功能作用尚不清楚。分离的大脑皮层细胞与CR-50共孵育重组培养时，野生型皮质细胞的基因依赖的组织发生组装与Reeler突变体相似。这些发现表明，Cajal-Retzius神经元上一个独特的分子的选择性表达对于哺乳动物新皮质皮质神经元的正常分层至关重要。
In the neurological mutant mouse reeler, the histological organization of the neocortex develops abnormally and essentially results in an inversion of the relative positions of the cortical layers. The reeler mutation, therefore, provides an insight into the molecular mechanisms underlying the formation of the cortical layers. We have generated a monoclonal antibody (CR-50) that probes a distinct allelic antigen present in wild-type but not in reeler mutant mice. CR-50 reacted specifically with Cajal-Retzius neurons, one of the first cortical neurons to differentiate in the neocortex, but whose functional role is not known. When dissociated cerebral cortical cells were incubated with CR-50 in reaggregation culture, the genotype-dependent histogenetic assembly of wild-type cortical cells resembled that of reeler mutants. These findings revealed that the selective expression of a distinct molecule on Cajal-Retzius neurons is critical for the normal lamination of cortical neurons in the mammalian neocortex.