Cytoplasmic deadenylation: regulation of mRNA fate.
Cytoplasmic deadenylation: regulation of mRNA fate.
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DOI：
10.1042/bst0381531
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发表时间：
2010-12
影响因子：
3.9
通讯作者：
Passmore LA
中科院分区：
文献类型：
--
作者：
Wiederhold K;Passmore LA
mRNA的poly（A）尾对基因表达的动力学有重要影响。一方面，它促进增强的mRNA稳定性以允许蛋白质的产生，即使在转录失活之后。另一方面，poly（A）尾的缩短（去腺苷酸化）通过诱导mRNA衰变而减慢或完全阻止mRNA的翻译。因此，去腺苷化在基因表达的转录后调控中起着至关重要的作用，决定了单个mRNA的命运。它既在基础mRNA周转中起作用，也在特定mRNA的时空调节翻译和衰变中起作用。在这里，我们讨论了真核生物中的mRNA去腺苷化，重点是主要的去腺苷酶，Ccr 4-Not复合物，包括其组成，调节和功能作用。
The poly(A) tail of mRNA has an important influence on the dynamics of gene expression. On one hand, it promotes enhanced mRNA stability to allow production of the protein, even after inactivation of transcription. On the other hand, shortening of the poly(A) tail (deadenylation) slows down translation of the mRNA, or prevents it entirely, by inducing mRNA decay. Thus deadenylation plays a crucial role in the post-transcriptional regulation of gene expression, deciding the fate of individual mRNAs. It acts both in basal mRNA turnover, as well as temporally- and spatially-regulated translation and decay of specific mRNAs. Here, we discuss mRNA deadenylation in eukaryotes, focusing on the main deadenylase, the Ccr4-Not complex, including its composition, regulation and functional roles.