miRNAs control HAM1 functions at the single-cell-layer level and are essential for normal embryogenesis in Arabidopsis
miRNAs control HAM1 functions at the single-cell-layer level and are essential for normal embryogenesis in Arabidopsis
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DOI：
10.1007/s11103-018-0719-8
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发表时间：
2018-03
影响因子：
5.1
通讯作者：
H. Takanashi;Hikari Sumiyoshi;Mirai Mogi;Y. Hayashi;Takayuki Ohnishi;N. Tsutsumi
中科院分区：
文献类型：
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作者：
H. Takanashi;Hikari Sumiyoshi;Mirai Mogi;Y. Hayashi;Takayuki Ohnishi;N. Tsutsumi
关键messagemiR171a控制HAM1功能的胚胎的原胚层细胞内，这些控制是必需的正常胚胎发生在Arabidopsis.AbstractArabidopsis thalianamiR171a是已知的绑定和切割的三个hairy MERISTEM（HAM）基因的mRNA编码的GRAS家族转录调节因子的成员。HAM基因在拟南芥中的分子功能仍有待进一步研究。然而，miR171a的详细表达模式以及miR171a抑制HAM基因失败的影响至今未知。在此，我们使用绿色荧光蛋白和共聚焦扫描显微镜显示了miR171 a和HAM 1的详细表达模式。结果表明，miR171 a在胚的表面细胞层和茎尖分生组织中表达，并控制HAM1的功能。为了确定miR171a抑制HAM1失败的影响，我们在HAM1基因的miR171a靶位点引入了7个同义突变（modifiedHAM1，mHAM1），并产生了具有由HAM1天然启动子驱动的admHAM1的转基因植物。转基因植株表现出器官发生缺陷。这些结果表明miR171a在单细胞层水平上对HAM1功能的控制对于拟南芥中适当的器官形成是必不可少的。
Key messagemiR171a controls HAM1 functions within the protodermal cells of the embryo, and these controls are essential for normal embryogenesis in Arabidopsis.AbstractArabidopsis thalianamiR171a is known to bind to and cleave mRNAs of threeHAIRY MERISTEM(HAM) genes that encode members of the GRAS family transcriptional regulators. The molecular functions of theHAMgenes are still being elucidated in Arabidopsis. However, detailed expression patterns ofmiR171aand the effects of the failure of miR171a to suppressHAMgenes were unknown till now. Here, we show the detailed expression patterns ofmiR171aandHAM1using green fluorescent protein and confocal scanning microscopy. Our observations revealed thatmiR171awas expressed in the surface cell layer of the embryo and shoot apical meristem, and it controlled HAM1 functions. To determine the impact of the failure of miR171a to suppress ofHAM1, we introduced seven synonymous mutations into the miR171a target site of theHAM1gene (modifiedHAM1, mHAM1) and generated transgenic plants that hadmHAM1driven byHAM1native promoter. ThemHAM1transgenic plants showed organogenic defects. These results indicate that the control of HAM1 functions at the single-cell-layer level by miR171a is essential for proper organ formation in Arabidopsis.