Phenotypic and toxicological characterization of toxic Nodularia spumigena from a freshwater lake in Turkey
Phenotypic and toxicological characterization of toxic Nodularia spumigena from a freshwater lake in Turkey
复制标题
DOI：
10.1016/j.hal.2008.06.007
复制
发表时间：
2009-01-01
期刊：
影响因子：
6.6
通讯作者：
Albay, Meric
中科院分区：
文献类型：
--
作者：
Akcaalan, Reyhan;Mazur-Marzec, Hanna;Albay, Meric
位于土耳其西部的淡水水体（盐度 = 0.5）伊兹尼克湖偶尔会观察到蓝藻水华。 2005 年夏季，湖中记录到了 Nodularia spumigena（尤尔根斯的 Mertens）。该物种的最大丝浓度（1.3 x 10(5) fil L-1）于 8 月测量，占蓝藻总丰度的 60%。毛状体是单生的、直的并且具有含有气泡的细胞。异形细胞在整个细丝中规则地间隔开。通过 HPLC、ELISA、PPIA 以及 LC-MS 检测分离的细丝中的球菌素。 HPLC 分析表明，培养的 N. spumigena 细胞中的重量分析球菌素浓度为每克干重 (d.w.) 578 p,g 球菌素。除了球藻素外，在球藻细胞提取物中还发现了去甲基化的球藻素变体。这是欧洲淡水湖中首次发现有毒的海绵猪笼草。 (C) 2008 Elsevier B.V. 保留所有权利。
Cyanobacterial blooms have been occasionally observed in Iznik lake, a freshwater body (salinity = 0.5) located in the western part of Turkey. Nodularia spumigena (Mertens in Juergens) was recorded in the lake in the summer months of 2005. Maximum filament concentration of the species (1.3 x 10(5) fil L-1) was measured in August and constituted 60% of total cyanobacteria abundance. Trichomes were solitary, straight and had cells containing gas vesicles. Heterocysts were regularly spaced throughout the filaments. In the isolated filaments nodularin was detected by HPLC, ELISA and PPIA as well as LC-MS. HPLC analysis showed that gravimetric nodularin concentration in cultured N. spumigena cells was 578 p,g of nodularin per gram dry weight (d.w.). Apart from nodularin, demethylated nodularin variant was also found in Nodularia cell extract. This is the first report of toxic N. spumigena in a European freshwater lake. (C) 2008 Elsevier B.V. All rights reserved.