A molecular level prototype for mechanoelectrical transducer in mammalian hair cells.
A molecular level prototype for mechanoelectrical transducer in mammalian hair cells.
复制标题
哺乳动物毛细胞中机电转换器的分子水平原型。
DOI：
10.1007/s10827-013-0450-z
复制
发表时间：
2013
影响因子：
1.2
通讯作者：
Wei,Guo-Wei
中科院分区：
文献类型：
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作者：
Park,Jinkyoung;Wei,Guo-Wei
机电换能器（MET）是哺乳动物听觉系统的重要组成部分。由于缺乏必要的分子构建模块，MET 通道的门控机制仍然是一个令人费解的问题，尽管有很多猜测。为了了解哺乳动物 MET 的工作原理，我们提出了一个分子水平原型，它构成了带电阻断剂、真实的离子通道及其周围的膜。为了验证所提出的原型，我们利用完善的离子通道理论，泊松-能斯特-普朗克方程，进行三维 (3D) 数值模拟。探索了各种模型参数，包括体离子浓度、施加的外部电压、阻断剂电荷和阻断剂位移，以了解所提出的 MET 原型的基本功能。我们表明，我们对响应阻断剂相对位移的通道开放概率的原型预测与大鼠耳蜗外毛细胞的实验观察非常一致。我们的结果似乎表明，连接发束的尖端连接门控 MET 通道。
The mechanoelectrical transducer (MET) is a crucial component of mammalian auditory system. The gating mechanism of the MET channel remains a puzzling issue, though there are many speculations, due to the lack of essential molecular building blocks. To understand the working principle of mammalian MET, we propose a molecular level prototype which constitutes a charged blocker, a realistic ion channel and its surrounding membrane. To validate the proposed prototype, we make use of a well-established ion channel theory, the Poisson–Nernst–Planck equations, for three-dimensional (3D) numerical simulations. A wide variety of model parameters, including bulk ion concentration, applied external voltage, blocker charge and blocker displacement, are explored to understand the basic function of the proposed MET prototype. We show that our prototype prediction of channel open probability in response to blocker relative displacement is in remarkable accordance with experimental observation of rat cochlea outer hair cells. Our results appear to suggest that tip links which connect hair bundles gate MET channels.