Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells
Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells
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DOI：
10.1016/j.leukres.2010.12.003
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Takahashi, Masuhiro
中科院分区：
文献类型：
--
作者：
Yamahira, Akie;Narita, Miwako;Takahashi, Masuhiro
建立了一株白血病浆细胞样树突状细胞系(PMDC05)，并在PMDC05中实现了pDC向MDCS的谱系内转化。在这篇文章中，我们展示了PMDC05在细胞免疫治疗中的适用性。在内毒素刺激下，PMDC05细胞表面抗原提呈相关分子的表达和细胞因子(IL-12p70和TNF-α)的产生均增强。PMDC05经脂多糖刺激后，其抗原提呈能力明显增强。将CD8(+)T细胞与脂多糖刺激的CD8(+)T细胞和WT1/CMVpp65多肽冲击的PMDC05共培养，可有效地产生WT1/CMVpp65四聚体(+)细胞毒T细胞。这些发现揭示了PMDC05在肿瘤和严重病毒感染的细胞免疫治疗中的适用性。(C)2010爱思唯尔有限公司。保留所有权利。
Establishment of a leukemia plasmacytoid dendritic cell line (PMDC05) and intra-lineage transformation from pDCs to mDCs in PMDC05 has been reported. In this paper, we show the applicability of PMDC05 for cellular immunotherapy. By stimulation with LPS, PMDC05 showed enhancement in expression of antigen presentation-associated surface molecules and production of cytokines (IL-12p70 and TNF-alpha). The antigen presenting ability was markedly increased in PMDC05 stimulated with LPS. By co-culturing of CD8(+) T cells with LPS-stimulated and WT1/CMVpp65 peptide-pulsed PMDC05, WT1/CMVpp65 tetramer(+) cytotoxic T lymphocytes were efficiently generated. These findings reveal the applicability of PMDC05 in cellular immunotherapy for tumor and severe viral infections. (C) 2010 Elsevier Ltd. All rights reserved.