Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp PCC 7942
Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp PCC 7942
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DOI：
10.1016/s1389-1723(04)70154-1
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发表时间：
2003-12-01
影响因子：
2.8
通讯作者：
Hirata, T
中科院分区：
文献类型：
--
作者：
Hamada, H;Kondo, Y;Hirata, T
蓝藻、聚球藻对柠檬烯和柠檬烯氧化物的生物转化。 PCC 7942，已被调查。 (S)-(+)-柠檬烯在其环内 C=C 双键的烯丙基位置上被蓝藻细胞立体和区域选择性羟基化为其相应的醇。这些细胞还表现出对(1S,2R,4R)-柠檬烯氧化物的环氧基团进行对映和立体选择性裂解以产生(1S,2S,4R)-柠檬烯-1,2-二醇的能力。使用 Ca2+-藻酸盐固定的蓝细菌细胞实现了从柠檬烯重复生产香芹醇。
The biotransformation of limonene and limonene oxide by the cyanobacterium, Synechococcus sp. PCC 7942, was investigated. (S)-(+)-Limonene was hydroxylated stereo- and regioselectively at its allylic position of the endocyclic C=C double bond by the cyanobacterial cells to its corresponding alcohol. The cells also showed the ability for the enantio- and stereoselective cleavage of the epoxide group of (1S,2R,4R)-limonene oxide to give (1S,2S,4R)-limonene-1,2-diol. The repetitive production of carveol from limonene was achieved using Ca2+-alginate-immobilized cyano-bacterial cells.