Proliferation and differentation of normal granulopoietic cells in continuous bone marrow cultures
Proliferation and differentation of normal granulopoietic cells in continuous bone marrow cultures
复制标题
连续骨髓培养中正常粒细胞的增殖和分化
DOI：
10.1002/jcp.1040930315
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发表时间：
1977
影响因子：
5.6
通讯作者：
E. Rabellino
中科院分区：
文献类型：
--
作者：
N. Williams;H. Jackson;E. Rabellino
报告了成功连续骨髓培养的改良条件，其中包括干细胞自我更新和粒细胞-巨噬细胞分化。发现培养数周的细胞与新鲜分离的骨髓细胞相同。来自培养物和原发性骨髓中性粒细胞的多态性中性粒细胞均显示C3和IgG受体，并且均主动吞噬外来颗粒。培养的和正常的CFU-c在对CFS的剂量反应性和沉降率特征方面都是相同的。
Modified conditions are reported for successful continuous bone marrow cultures with stem cell self‐renewal and granulocyte‐macrophage differentiation. Cells cultured over several weeks were found to be identical to freshly isolated bone marrow cells. Polymorphic neutrophils derived from cultures and primary bone marrow neutrophils both showed C3 and IgG receptors and both actively phagocytosed foreign particles. Cultured and normal CFU‐c were identical, both in their dose responsiveness to CFS and in their sedimentation rate characteristics.