Comparison of lymphocyte immune phenotypes in bronchoalveolar lavage of non-smoking patients with sarcoidosis and other interstitial lung diseases
Comparison of lymphocyte immune phenotypes in bronchoalveolar lavage of non-smoking patients with sarcoidosis and other interstitial lung diseases
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DOI：
10.21037/jtd.2019.06.05
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发表时间：
2019-06-01
影响因子：
2.5
通讯作者：
Petrek, Martin
中科院分区：
文献类型：
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作者：
Novosadova, Eva;Navratilova, Zdenka;Petrek, Martin
背景：支气管肺泡灌洗(BAL)作为辅助检查方法在间质性肺疾病的诊断中仍被作为辅助手段。吸烟已经被描述为影响BAL，灌洗细胞的轮廓。据我们所知，到目前为止，很少有报道研究非吸烟结节病患者的CD_3(+)CD_4(+)和CD_3(+)CD_8(+)淋巴细胞亚群，并将其与其他非吸烟的间质性肺疾病(ILDS)患者进行比较。方法：我们比较了包括肺结节病(S)、特发性肺纤维化(IPF)(n=22)在内的非吸烟患者(n=297)的淋巴细胞免疫表型及其与CD_3(+)、CD_3(+)CD_4(+)和CD_3(+)T_8(+)细胞标志物的差异。过敏性肺炎(HP)15例，其他间质性特发性肺炎(OIIP)39例。根据预后将S患者分为4组：18例胸片Lofgren综合征(LS)患者和3例非吸烟者IPF。结论：非吸烟者结节病具有典型的BAL细胞型特征。BAL的细胞学特征有助于鉴别不太严重的结节病。其明确的诊断价值应作为进一步临床研究的主题，考虑到影响BAL的其他临床因素，如吸烟或治疗等，对大量特征良好的患者进行研究。
Background: Bronchoalveolar lavage (BAL) as complementary method is still used as ancillary tool in diagnosis of interstitial lung diseases. lbbacco smoking has been described to affect the BAL, lavage cellular profile. lb our knowledge, only few reports have so far investigated CD3(+)CD4(+) and CD3(+)CD8(+) lymphocyte subsets in non-smoking sarcoidosis patients additionally stratified according to CXR stage, and compared them to other non-smoking patients with interstitial lung diseases (ILDs).Methods: We compared lymphocytes immune phenotypes, subsets, with CD3(+), CD3(+)CD4(+) and CD3(+)TD8(+) cell markers, in the non-smoking subjects (n=297) including the patients with pulmonary sarcoidosis (S), idiopathic pulmonary fibrosis (IPF) (n=22), hypersensitivity pneumonitis (HP) (n=15), other interstitial idiopathic pneumonias (OIIPs) (n=39). According to prognosis, the patients with S were divided into four groups: 18 patients with Lofgren's syndrome (LS) in chest X-ray (CXR) = 3) and the non-smokers with IPF.Conclusions: Our data on the non-smokers confirmed the presence of the typical BAL cellular profile in sarcoidosis. The BAL cellular profile was helpful namely for differentiation of less advanced sarcoidosis. Its definite diagnostic utility should be the subject of further clinical studies with large numbers of the well characterized patients taking into consideration other clinical factors influencing BAL, cellular profile, such as smoking or treatment.