Thymol Isolated from Thymus vulgaris L. Inhibits Colorectal Cancer Cell Growth and Metastasis by Suppressing the Wnt/β-Catenin Pathway.
Thymol Isolated from Thymus vulgaris L. Inhibits Colorectal Cancer Cell Growth and Metastasis by Suppressing the Wnt/β-Catenin Pathway.
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从百里香中分离出的百里酚通过抑制 Wnt/β-连环蛋白途径来抑制结直肠癌细胞的生长和转移。
DOI：
10.2147/dddt.s254218
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发表时间：
2020
影响因子：
4.8
通讯作者：
Wenjing Zhang
中科院分区：
文献类型：
--
作者：
Qiongyao Zeng;Yuncheng Che;Yu Zhang;Mei Chen;Qiang Guo;Wenjing Zhang
结直肠癌（CRC）是最常见的癌症之一，发病率和死亡率很高。然而，目前尚无安全有效的治疗方法。百里酚是一种酚类化合物，被认为可安全用于食品以及医疗和化妆品领域。越来越多的证据表明，百里酚对多种癌症（包括结直肠癌）具有显着的抗肿瘤作用。然而，百里酚如何对结直肠癌产生这些影响以及相关的潜在机制仍不清楚。用不同浓度的百里酚处理HCT116和Lovo细胞。细胞计数试剂盒-8 (CCK-8) 和 Transwell 迁移和侵袭测定分别用于评估细胞增殖、迁移和侵袭。通过流式细胞术测量细胞凋亡和细胞周期分布。采用RT-qPCR、Western blot和免疫组织化学方法检测相关基因及其蛋白产物的表达。在本研究中，我们检测了从中药百里香中提取的百里酚的抗肿瘤活性。我们发现百里酚体外处理可抑制CRC细胞增殖并诱导细胞凋亡和细胞周期阻滞。此外，用 75 和 150 mg/kg 百里香酚进行体内治疗可导致肿瘤体积显着减小。百里酚通过激活 BAX/Bcl-2 信号通路诱导 CRC 细胞凋亡。此外，在体外和体内，百里酚通过抑制 Wnt/β-catenin 通路的激活，抑制 CRC 细胞上皮间质转化 (EMT)、侵袭和转移。百里酚可能通过抑制 Wnt/β-catenin 信号通路来阻止 CRC 进展，突出了其作为治疗 CRC 的新型治疗选择的潜力。
Colorectal cancer (CRC) is one of the most commonly occurring cancers and is associated with high morbidity and mortality. Nevertheless, there is currently no safe and effective treatment for this condition. Thymol is a phenolic compound that is recognized as safe for use in food as well as medical and cosmetic fields. Increasing evidence has indicated that thymol exerts prominent antitumor effects in a variety of cancers, including CRC. However, how thymol elicits these effects on CRC and the associated underlying mechanisms remains unclear.HCT116 and Lovo cells were treated with different concentrations of thymol. Cell Counting Kit-8 (CCK-8) and transwell migration and invasion assays were used to evaluate cell proliferation, migration, and invasion, respectively. Cell apoptosis and cell cycle distribution were measured by flow cytometry. RT-qPCR, Western blot, and immunohistochemistry were used to detect the expression of related genes and their protein products.In this study, we tested the antitumor activity of thymol extracted from a Chinese medicinal herb, Thymus vulgaris L. We show that thymol treatment in vitro inhibited cell proliferation and induced apoptosis and cell cycle arrest in CRC. Furthermore, in vivo treatment with 75 and 150 mg/kg thymol led to a significant decrease in tumor volume. Thymol administration induced CRC cell apoptosis through activation of the BAX/Bcl-2 signaling pathway. In addition, thymol suppressed CRC cell epithelial-mesenchymal transition (EMT), invasion, and metastasis via inhibiting the activation of the Wnt/β-catenin pathway, both in vitro and in vivo.Thymol may prevent CRC progression through inhibition of the Wnt/β-catenin signaling pathway, highlighting its potential as a novel therapeutic option for the treatment of CRC.