Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
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泛素阴性、嗜酸性神经元细胞质内含物与应激颗粒和自噬相关：对两例病例的免疫组织化学研究。
DOI：
10.1111/neup.12075
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发表时间：
2014
期刊：
影响因子：
2.3
通讯作者：
Wakabayashi K.
中科院分区：
文献类型：
--
作者：
Mori F;Watanabe Y;Miki Y;Tanji K;Eto K;Wakabayashi K.
鉴定病理性包涵体的蛋白质成分是了解相关疾病机制的重要步骤。我们对两个先前报道的嗜酸性神经元胞质包涵体（NCIs）病例进行了化学染色检查（病例1，Moriet al. Neuropathology 2010; 30：648-53;病例2，Kojimaet al. Acta Pathol Jpn 1990; 40：785-91），使用针对与细胞骨架成分、泛素-蛋白酶体系统、自噬-溶酶体途径和应激颗粒形成相关的蛋白质的67种抗体。例1中嗜酸性粒细胞NCI的区域分布模式与例2中有显著不同。然而，两种情况下的NCIs对泛素和p62呈免疫阴性，对应激颗粒标志物以及自噬相关蛋白（包括含valosin蛋白）呈免疫阳性。考虑到真核细胞应激颗粒是通过自噬和含valosin的蛋白功能清除的，我们的研究结果表明，目前两种情况下的嗜酸性NCIs可能代表了应激颗粒的自噬清除过程。
Identification of the proteinaceous components of the pathological inclusions is an important step in understanding the associated disease mechanisms. We immunohistochemically examined two previously reported cases with eosinophilic neuronal cytoplasmic inclusions (NCIs) (case 1, Moriet al. Neuropathology 2010; 30: 648–53; case 2, Kojimaet al. Acta Pathol Jpn 1990; 40: 785–91) using 67 antibodies against proteins related to cytoskeletal constituents, ubiquitin‐proteasome system, autophagy‐lysosome pathway and stress granule formation. Regional distribution pattern of eosinophilic NCIs in case 1 was substantially different from that in case 2. However, NCIs in both cases were immunonegative for ubiquitin and p62 and were immunopositive for stress granule markers as well as autophagy‐related proteins, including valosin‐containing protein. Considering that eukaryotic stress granules are cleared by autophagy and valosin‐containing protein function, our findings suggest that eosinophilic NCIs in the present two cases may represent the process of autophagic clearance of stress granules.