A CNS specific proteoglycan associated with astrocytes in rat optic nerve.
A CNS specific proteoglycan associated with astrocytes in rat optic nerve.
复制标题
与大鼠视神经星形胶质细胞相关的中枢神经系统特异性蛋白多糖。
DOI：
10.1016/0006-8993(92)90526-f
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Bidanset,DJ
中科院分区：
文献类型：
--
作者：
GeisertJr,EE;Williams,RC;Bidanset,DJ
单克隆抗体 (mAb) TED15 鉴定出一种新型中枢神经系统特异性硫酸角质素蛋白多糖。该抗体可识别角蛋白酶消化后从核心蛋白中去除的碳水化合物表位。用肝素酶、肝素酶或软骨素酶 ABC 消化后，糖蛋白的相对分子量不会改变。通过免疫印迹分析，TED15抗原在间脑衍生物中存在差异性分布，存在于视神经中但不存在于视网膜中。在视神经内，TED15 抗原与星形胶质细胞相关，而在视网膜中未观察到星形胶质细胞或穆勒细胞的标记。
A novel CNS specific keratan sulfate proteoglycan is identified by a monoclonal antibody (mAb) TED15. The antibody recognizes a carbohydrate epitope that is removed from the core protein following keratanase digestion. The relative molecular weight of the glycoprotein is not altered following digestion with heparinase, heparitinase or chondroitinase ABC. By immunoblot analysis, the TED15 antigen is differentially distributed in diencephalic derivatives, being present in the optic nerve but absent from the retina. Within the optic nerve, the TED15 antigen is associated with astrocytes, while in the retina no labeling of astrocytes or Müller cells is observed.