OsOFP19 modulates plant architecture by integrating the cell division pattern and brassinosteroid signaling
OsOFP19 modulates plant architecture by integrating the cell division pattern and brassinosteroid signaling
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OsOFP19 通过整合细胞分裂模式和油菜素类固醇信号传导来调节植物结构
DOI：
10.1111/tpj.13793
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发表时间：
2018
期刊：
影响因子：
7.2
通讯作者：
Li Jianxiong
中科院分区：
文献类型：
--
作者：
Yang Chao;Ma Yamei;He Yong;Tian Zhihong;Li Jianxiong
OVATE家族蛋白（OFPs）的特性揭示了它们通过与不同类型的转录因子相互作用发挥功能。然而，对这些过程的分子基础了解甚少。在这里，我们报道了OsOFP19负调控油菜素内酯（BR）反应，并将其与细胞分裂模式结合起来，通过与DWARF and LOW - TILLERING （DLT）和oryza sativahomeobox1 （OSH1）相互作用影响植物结构，包括籽粒形状。osofp19的过表达导致了叶片较厚、茎和根较强的半矮化，这是由于表皮下组织中细胞层的增加所致。进一步的研究表明OsOFP19与OSH1相互作用，这种相互作用增强了这些蛋白的转录活性，并导致细胞从斜向周分裂的转变。此外，DLT与OsOFP19和OSH1相互作用，并在这两种相互作用中作为拮抗剂。因此，OsOFP19、OSH1和DLT组成了一个功能复合物，在植物生长发育过程中，在调节BR信号和决定细胞分裂模式中起着关键作用。
Characterization of OVATE family proteins (OFPs) has revealed that they exert functions by interacting with different types of transcription factor. However, the molecular bases of these processes are poorly understood. Here, we report that OsOFP19 negatively modulates brassinosteroid (BR) response and integrates it with the cell division pattern to affect plant architecture, including grain shape, through interaction with both DWARF AND LOW‐TILLERING (DLT) andOryza sativahomeobox1 (OSH1). Overexpression ofOsOFP19caused a semi‐dwarf stature with thicker leaves and stronger culms and roots, which result from an increase in cell layers in the sub‐epidermal tissue. Further studies revealed that OsOFP19 interacts with OSH1, and that this interaction mutually enhances the transcriptional activity of these proteins and leads to a transition from anticlinal to periclinal cell division. Furthermore, DLT interacts with both OsOFP19 and OSH1 and acts as an antagonist in the two interactions. Therefore, OsOFP19, OSH1 and DLT form a functional complex which plays a pivotal role in modulating BR signaling and determining the cell division pattern during plant growth and development.