Changes in blood and spleen lymphocyte populations following antigen challenge in immature male chickens
Changes in blood and spleen lymphocyte populations following antigen challenge in immature male chickens
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DOI：
10.1080/00071669608417911
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发表时间：
1996-09-01
影响因子：
2
通讯作者：
Siegel, HS
中科院分区：
文献类型：
--
作者：
Trout, JM;Mashaly, MM;Siegel, HS
1.研究了抗原（Ag）注射对Cornell K系公鸡淋巴细胞群分布的影响.进行了两个实验。在第一个实验中，鸡被注射了牛种布氏杆菌（BA），这是一种据称的T-非依赖性抗原。在第二个实验中，鸡被注射了绵羊红细胞（SRBC），一种T依赖性抗原。分别于注射Ag后0、3、6、9、12和24 h分离外周血淋巴细胞（PBL）和脾淋巴细胞，用单克隆抗体（mAb）检测B淋巴细胞、CD 4（+）和CD 8（+）细胞.注射BA或SRBC后，血液或脾脏中的B淋巴细胞均无明显变化。相反，注射BA和SRBC后，血液中的CD 4（+）细胞减少，脾脏中的CD 4（+）细胞增加。注射BA后血液和脾脏中的CD 8（+）细胞均减少，但注射SR 8 C后血液和脾脏中的CD 8（+）细胞均无变化.这些结果表明，有一个变化，脾和循环淋巴细胞群，特别是T辅助细胞，以下Ag注射。辅助性T细胞显然是参与体液免疫启动的主要群体。
1. The effects of antigen (Ag) injection on the distribution of lymphocyte populations of Cornell K-strain male chickens were studied.2. Two experiments were conducted. In the first, chickens were injected with Brucella abortus (BA), a purported T-independent antigen. In the second, chickens were injected with sheep red blood cells (SRBC), a T-dependent antigen. Peripheral blood lymphocytes (PBL) and spleen lymphocytes isolated at 0, 3, 6, 9, 12 and 24 h following Ag injection were stained with monoclonal antibodies (mAb) detecting B-lymphocytes, CD4(+) and CD8(+) cells.3. B-lymphocytes in the blood or spleen showed no significant changes following either BA or SRBC injection. In contrast, CD4(+) cells were decreased in the blood and increased in the spleen following BA and SRBC injections. CD8(+) cells were decreased in both blood and spleen following BA injection but were unchanged in either blood or the spleen following SR8C injection.4. These results indicate that there is a change in both spleen and circulating lymphocyte populations, especially T-helper cells, following Ag injection. T-helper cells are apparently the primary population involved in the initiation of humoral immunity.