HLA-B*35-PX and HLA-B*35-PY subtype differentiation does not predict observed differences in level of HIV control in a Peruvian MSM cohort.
HLA-B*35-PX and HLA-B*35-PY subtype differentiation does not predict observed differences in level of HIV control in a Peruvian MSM cohort.
复制标题
HLA-B*35-PX 和 HLA-B*35-PY 亚型分化并不能预测秘鲁 MSM 队列中观察到的 HIV 控制水平差异。
DOI：
10.1097/qad.0000000000000403
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Brander,Christian
中科院分区：
文献类型：
--
作者：
Olvera,Alex;Ganoza,Carmela;Pérez-Álvarez,Susana;Hildebrand,William;Sanchez,Jorge;Brander,Christian
Fig. 1.秘鲁MSM人群中病毒载量分布、显性HLA-B35亚型限制性T细胞应答和等位基因序列多态性(a)显示了分类为PX、PY或未分类等位基因的HLAIB 35亚型等位基因和表达这些等位基因的个体中的中值血浆病毒载量（VL）。VL具有统计学显著差异的等位基因由灰色条表示（P <0.05）;群体中值VL由虚线表示。各种亚型的群体等位基因频率由条形指示。(b)VL的HLA-Bo 35等位基因分类为PX，PY和未分类的个体相比，没有携带HLA-Bo 35亚型等位基因。使用单因素方差分析比较VL，使用Student t检验进行两两比较（P <0.001，P <0.01，P <0.05）。(c)存在的HLA-Bo 35亚型的氨基酸比对中的可变位置。位置114和116以灰色阴影表示，作为显示显著不同VL的唯一多态性（P <0.05）。HLA亚型序列信息从http：//www. ebi AC. uk/ipd/imgt/hla/allele。HTML. (d)使用HEPITOPE算法（http：//www.heptope.com）确定对特定OLP和HLA-Bo 35亚型的反应之间的关联。艾滋病。兰勒gov/content/immunology/hepitopes），并使用ELF（http：//www.艾滋病。兰勒gov/content/sequence/ELF/epitope_analyzer. html）。每个HLA-Bo 35亚型等位基因的OLP反应曲线显示为单侧Fisher精确检验P <0.01的反应评分用黑色标记，相关性为0.01 <P <0.05的反应评分用灰色标记。底线表示与同一OLP具有反应性的等位基因的数量。仅指示由至少两个HLA等位基因共享的对OLP的应答。
Fig. 1. Viral load distribution, dominant HLA-B35 subtype restricted T-cell responses and allele sequence polymorphisms in the Peruvian MSM cohort.(a) HLAÃB35 subtype alleles classified as PX, PY or unclassified alleles and the median plasma viral load (VL) in individuals expressing these alleles are shown. Alleles with statistically significant differences in VL are indicated by grey bars (P< 0.05); population median VL is indicated by a dotted line. Population allele frequency of the various subtypes is indicated by bars.(b) VL of HLA-BÃ35 alleles classified as PX, PY and unclassified compared with individuals bearing no HLA-BÃ35 subtype alleles. VLs were compared using one-way ANOVA and two-by-two comparisons using Student’s t-test (ÃÃÃP< 0.001, ÃÃ P< 0.01, ÃP< 0.05).(c) Variable positions in an amino acid alignment of the HLA-BÃ35 subtypes present. Positions 114 and 116 are shaded in gray as the only polymorphisms that showed significantly different VL (P< 0.05). HLA subtype sequence information was obtained from http://www. ebi. ac. uk/ipd/imgt/hla/allele. html.(d) Associations between responses to particular OLP and HLA-BÃ35 subtypes were determined using the HEPITOPE algorithm (http://www. hiv. lanl. gov/content/immunology/hepitopes) and the presence of defined epitopes in the OLP sequence was revised using ELF (http://www. hiv. lanl. gov/content/sequence/ELF/epitope_analyzer. html). The OLP response profile is shown for each HLA-BÃ35 subtype allele with responses scoring with a one-sided Fisher’s exact test of P< 0.01 marked in black and those with an association of 0.01< P< 0.05 in gray. The bottom line indicates the numbers of alleles that share reactivities to the same OLP. Only responses to OLP shared by at least two HLA alleles are indicated.